101 things you (maybe) didn’t know about MacVector: #28 – Identifying Methylation Blocked Restriction Sites

A big thanks to Jeffrey Dvorin at Boston Children’s Hospital for this great suggestion. Most common laboratory strains of E. coli contain a number of methylase enzymes that modify DNA residues, preventing certain restriction enzymes from cutting DNA isolated from those strains. The two most relevant enzymes are the Dam methylase that methylates the A […]

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101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson Assemblies

We’ve had a few MacVector users ask us recently if MacVector can be used to create constructs using the increasingly popular “Gibson Assembly” method. For those who are not familiar with this method, it was first described in 2009 by Daniel Gibson of the J. Craig Venter Institute. They showed that you can assemble multiple […]

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