How to quickly design primers to amplify a feature on your sequence

To design a pair of primers to amplify a single feature is pretty quick with MacVector. Select a feature in the MAP tab Run Primer Design (Primer3) Ensure the dropdown menu is set to AMPLIFY FEATURE Click OK Check the summary shows that primers have been found and select the spreadsheet and graphical view Click […]

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Quickly checking a small sequencing project

For analysing large sequencing datasets, whether de novo or mapping reads against a reference you need Assembler. However, many times you do not need a powerful tool but just a quick way to check some sequencing data. For example for checking small sequencing projects, such as a site directed mutagenesis, looking for SNPs in a […]

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101 things you (maybe) didn’t know about MacVector: #42 – Managing segmented features

If you download eukaryotic genomes from GenBank, you will find that many coding (i.e. CDS) features are segmented and consist of multiple individual segments joined together into a single long feature. You can see an example of this with the human cystic fibrosis transmembrane regulator gene – you find a copy of this in the […]

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Upgrade MacVector with a 30% discount during April

Don’t be an April Fool, upgrade with a…. 30% discount … before MacVector 14 is released in May. This is no April Fool’s joke. Here’s a great way to upgrade your copy of MacVector before the release of MacVector 14 next month. For the entire month of April you will get a 30% discount on […]

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