General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Comparing coverage depth across multiple reference assemblies with MacVector 17

We’re in the final stages of testing our latest release, MacVector 17, which will be released in January.  Here’s a preview of another one of the new tools within our most feature packed release yet.

MacVector has a greatly improved Assembly Projects manager, for better organization of multiple sequencing datasets, multiple references sequences and repeated jobs. Every time you run a new assembly job (either a reference assembly or de novo). A new job object is created in the Assembly Project window contains resulting contigs and any unaligned reads from the assembly.

A new properties tab lists all of the relevant details for the currently selected job, including input parameters, total assembled reads, average contig length, total contig length and N50 to give you an idea of the quality of genome assemblies.

A new Coverage tab directly compare multiple reference assemblies. You can compare different sequencing datasets assembled against the same reference sequence with expression level comparison. Now that you can store multiple assembly jobs in a single Assembly Project, you can also directly compare multiple runs of the same dataset against a single reference sequence to compare which is the best assembly.

MacVector 17 will be released during January 2019. It contains brand new tools for comparing genomes, making restriction enzyme cloning easier, automated design of Gibson/LIC cloning strategies, highlighting assembly issues, comparing multiple sequence datasets against a single reference and making plasmid maps even more beautiful, by displaying your own primer binding sites! Finally MacVector 17 supports macOS Mojave’s Dark Mode to aid concentration on those late night primer design sessions!

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