Author Archives: Chris

Identifying transposon insertion sites from multiplexed NGS data

Transposon mutagenesis is a common approach for investigating gene function in bacterial genomes by selecting for clones where the transposon inserting into the genome has generated a specific phenotype. You can then simply sequence the entire genome of each clone by NGS to identify the transposon insertion site. To lower the cost of such experiments, […]

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Human Transcriptome RNA-Seq Analysis Using MacVector

With MacVector Pro and Assembler you can use Bowtie to perform RNA-Seq analyses using NGS data. The interface even has specialized output tabs listing the coverage information and statistics for each annotated CDS and gene feature on the genome. You can download a short tutorial and a sample dataset that illustrate the analysis workflow using […]

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Use a right-click in the Editor tab to see if your contig can be circularized

MacVector incorporates no less than THREE different de novo assemblers, phrap, velvet and SPAdes. While all are great assemblers, with each having their own specific advantages, none of them will generate a circular sequence from input reads. However, MacVector also includes a tool to help you with this. If you are assembling reads representing plasmid […]

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Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features

There are many genomes in the Genbank database that cannot be downloaded as single annotated sequences. These might be large multi-chromosome eukaryotic genomes, but, increasingly, partially sequenced bacterial chromosomes where the major contigs have been annotated using the NCBI annotation pipeline. Typically, when you encounter these, there are options to download annotated versions of these […]

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Opening Genbank or FASTA files with multiple sequences as individual sequences

Many sequence formats contain multiple concatenated sequence entries. For example FASTA and Genbank are two formats capable of storing multiple individual sequences. By default MacVector will treat such sequences as alignments and open them in the Multiple Sequence Alignment editor. Most users who want to open such a file do want to see an alignment. […]

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Optimizing Align To Folder Parameters for use with NGS Data

You can use the Database | Align To Folder function to scan large fasta or fastq files containing NGS data to find and retrieve just those reads that match a specific target sequence. The search is aware of paired-end reads, so when you retrieve hits, both reads of a pair will be saved into a […]

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MacVector and macOS Catalina

The next OS release for the Mac will arrive during September. macOS Catalina is a major OS release and includes many new features. As usual our developers have been hard at work ensuring that when macOS Catalina is released, MacVector 17 will be fully compatible. For older versions you can check compatibility on our website […]

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Upgrade to the most feature packed version of MacVector yet and install on multiple Macs with a 50% discount.

Personal licenses are ideal for using on a single Mac, but not if you have multiple Macs or want to install on a shared lab computer as well as your personal Mac. So why not upgrade to a standard license of MacVector Pro 17 that you can share among a group of users in the […]

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The MacVector Team will be at ASM Microbe 2019 in San Francisco.

We’re at ASM Microbe 2019 in San Francisco from Friday 21st June until Sunday 23rd. The show is finally back on the West Coast at the Moscone Convention Center after 6 years on the East Coast and New Orleans. This year the exhibit hall hours are unchanged from last year’s show: Friday, June 21st – […]

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What can MacVector do for my lab?

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Compare Genomes: Compares two related annotated genomes to identify identical, similar and weakly similar features. Sequence assembly of NGS data […]

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