Comments for MacVector talk

Comment on Gateway and TOPO Cloning with MacVector by Emre

Emre — Fri, 09 Sep 2011 12:48:07 +0000

Dear Chris,

Neither any of the files that you uploaded recently nor the original RE files that come wtih MacVector has attP sequences on them. attB 1-4 are there but not the attP except for attP5. And attL4 is not there either. Is there a specific reason for this?

Thanks

Emre

Comment on Gateway and TOPO Cloning with MacVector by Chris

Chris — Mon, 15 Aug 2011 13:14:44 +0000

Here's an updated Gateway file

Comment on Gateway and TOPO Cloning with MacVector by Chris

Chris — Sun, 14 Aug 2011 14:29:54 +0000

Hi Jason. Thanks for your comment. There was a bug in the file that caused the reverse complement site to not be recognised. That’s now fixed. I’ll upload the file shortly

Comment on Gateway and TOPO Cloning with MacVector by Jason

Jason — Thu, 11 Aug 2011 18:53:57 +0000

I am having problems with MacVector not recognizing attB2 sites all the time. If cut this sequence:
GGGGACCACTTTGTACAAGAAAGCTGGGTN
it shows an attB2 site. If I then “reverse complement” the same sequence, it no longer shows an attB2 site.

Any suggestons

Comment on Gateway and TOPO Cloning with MacVector by Chris

Chris — Mon, 08 Aug 2011 14:22:37 +0000

Hi Emre, it sounds like that file has lost its resource fork. Try using this file.

Comment on Gateway and TOPO Cloning with MacVector by Emre

Emre — Wed, 20 Jul 2011 13:02:34 +0000

Hello,

Thanks for the quick response.

I downloaded the file and placed it in the “Restriction Enyzme” folder. While it is possible to make a Restriction Enzyme analysis using the updated file, the files is grayed out when I try to select it in the Automatic RE Analysis feature under Map View. The same is true with the other file you provided previously. Any tricks that I can try?

Thanks

Comment on Gateway and TOPO Cloning with MacVector by Kevin Kendall

Kevin Kendall — Wed, 20 Jul 2011 13:01:45 +0000

You can select multiple sites for copying by drag-selecting, you just can’t command-select or shift-select in MacVector 12 and earlier. As it happens, we’ve rewritten the Restriction Editor window for our upcoming MacVector 12.5 release and it now uses a standard OS X Cocoa TableView, so you get all the usual selection behavior you’d expect in a normal list.

Comment on Gateway and TOPO Cloning with MacVector by admin

admin — Wed, 20 Jul 2011 12:40:52 +0000

Hi Nils, here you go. Incidentally you can copy and paste single sites between RE files as long as they are unlocked.

Comment on Gateway and TOPO Cloning with MacVector by Emre

Emre — Wed, 20 Jul 2011 12:28:44 +0000

Hi Chris,

Is there an easy way to integrate this updated Gateway file to the common enzymes file? Doing it one by one would be tedious.

Thanks

Comment on Gateway and TOPO Cloning with MacVector by Chris

Chris — Wed, 08 Jun 2011 07:50:42 +0000

Hi Nils,

there’s an updated “Gateway” file available for download.
Get this then place it in your “Restriction Enzyme” folder.