How to design a digest to screen minipreps after a ligation.

MacVector’s Agarose Gel tool can be used to quickly design a restriction digest to screen minipreps following a ligation.  (View full size on website…) Replicate your ligation in MacVector. Select the two sites, for subcloning your targeted gene, and click DIGEST. Drag the digested fragment from the Cloning Clipboard to your vector click LIGATE. Create […]

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MacVectorTip: Trimming by Quality in sequence assemblies

Many of our users may be familiar with the ability of Sequencher to semi-automatically trim poor quality sequences from the ends of Sanger ABI reads. Although it is generally not necessary to do this in MacVector because most of the algorithms can automatically handle poor quality data, there are times when it can be beneficial. […]

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