Visualising ORF analysis results in the MAP tab.

The Map tab of MacVector is a powerful way to visualise and interact with your sequences. All analysis tools will work directly in the Map tab. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more. In fact one of the only tasks, you will need […]

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Cloning Clipboard: Documenting the history of a construct

Designing and documenting cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. Not only that but every step is documented and recorded in the resulting sequence, so you always know where each fragment came from. Ligating a single fragment into a vector is as […]

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101 things you (maybe) didn’t know about MacVector: #47 – Optimal PCR Annealing Temperature

I wrote about how MacVector calculates the melting temperature of a primer in an earlier blog post. One other common question we get is, “once I have designed a pair of primers, what is the optimum annealing temperature to use in the PCR?” MacVector calculates this and displays the results in the Analyze | Primer […]

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