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Use a right-click in the Contig Editor tab to see if your contig can be circularized
Read more: Use a right-click in the Contig Editor tab to see if your contig can be circularizedMacVector 16 incorporates no less than THREE different de novo assemblers, phrap, velvet and SPAdes. While all are great assemblers, with each having their own specific advantages, none of them will generate a circular sequence from input reads. However, MacVector 16 also includes a new feature to help you with this. If you are assembling…
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Downloading hits from the MacVector BLAST Map results tab
Read more: Downloading hits from the MacVector BLAST Map results tabMacVector’s BLAST Map results tab (added in MacVector 15.5) is a unique interface for examining the annotations around hits to a query sequence. Each pane in the display represents a High Scoring Segment Pair, as seen in the BLAST Aligned Sequence tab. At the lower left corner of each pane is a Download button –…
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Turning on/off the SCAN FOR missing Features and ORFs
Read more: Turning on/off the SCAN FOR missing Features and ORFsIf you’re running MacVector 15.5 or later then you will have noticed extra features annotated to your sequences. These are from the Scan for ORFs tool (added in MacVector 15.5) and the Scan For Missing Features (added in MacVector 16) tools that automatically scan every DNA sequence window for open reading frames, missing annotations and…
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Controlling The Automatic ORF Display in MacVector 15.5
Read more: Controlling The Automatic ORF Display in MacVector 15.5MacVector automatically scans every DNA sequence window for open reading frames and displays the results in the Map tab. The setting for this are controlled by the MacVector | Preferences -> DNA Map pane, along with the automatic Show restriction sites settings. The Minimum Number of Codons setting is fairly obvious. 5’ ends are starts…
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Use the BLAST Map to better identify blast hits
Read more: Use the BLAST Map to better identify blast hitsWith the advent of cheap Next Generation Sequencing technologies, there has been an explosion of whole genome sequences deposited in BLAST databases. One consequence of this is that, particularly for sequences of bacterial origin, most of the significant hits are to entire genomes. The classic BLAST results show the sequence alignments, but give no indication…
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MacVector’s Scan For Missing Features tool makes beautiful plasmid maps easy!
Read more: MacVector’s Scan For Missing Features tool makes beautiful plasmid maps easy!Scan for.. Missing Features: Sequences are automatically scanned and missing features displayed. A simple right-click converts them to a permanent feature. Even blank sequences will be displayed fully annotated with common features. You can even add your own proprietary features.
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Testing pairs of primers with MacVector
Read more: Testing pairs of primers with MacVectorMacVector’s Primer Design tools makes it easy to test your primers. You can insert them directly from MacVector’s Primer Database or copy and paste them.
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How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.
Read more: How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.
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Annotating a gene in MacVector
Read more: Annotating a gene in MacVectorCreating biologically accurate AND beautiful maps of your plasmids is easy in MacVector. All you need to do is select some sequence, then open the FEATURES EDITOR. Every annotation is fully compatible with the Genbank specification and Feature Table.
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Downloading hits from the MacVector 15.5 BLAST Map results tab
Read more: Downloading hits from the MacVector 15.5 BLAST Map results tabThe MacVector 15.5 BLAST Map results tab is a unique interface for examining the annotations around hits to a query sequence. Each pane in the display represents a High Scoring Segment Pair, as seen in the BLAST Aligned Sequence tab. At the lower left corner of each pane is a Download button – when you…
