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MacVector and Applescript
Read more: MacVector and ApplescriptUp to MacVector 13 MacVector has had limited Applescript support. The current release (12.7.5) is able to open sequences, print them and that’s about it! We’ve had frequent requests for MacVector to batch process files. So with the new release (coming very soon) you can batch process files. No analysis can be undertaken just yet…
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MacVector 12.7 Training Workshop, LMB, Cambridge
Read more: MacVector 12.7 Training Workshop, LMB, CambridgeWhen: Thursday 19th September, 2013, 2:00 – 4:00, Where: Max Perutz Lecture Theatre, LMB Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the past two years to MacVector.…
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Using Blast and Entrez in MacVector with a proxy server
Read more: Using Blast and Entrez in MacVector with a proxy serverBlast and Entrez connect to the NCBI server using the normal “http” ports (exactly the same as if it was a web browser). If a web browser can access the NCBI’s server then MacVector should be able to. The address for the Blast server is: http://www.ncbi.nlm.nih.gov:80/blast/Blast.cgi However, if you get the following error message then…
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Removing a restriction site by linearising and religating a plasmid
Read more: Removing a restriction site by linearising and religating a plasmidBeing able to remove a restriction site by digesting to linearise a plasmid, Klenow treating then religating the linear fragment is a very useful trick and an often requested feature. This is now possible with the new Cloning Clipboard in MacVector 12.7. A new circular sequence will be created from your digested fragment. The origin…
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Assembler: Using the coverage map of the Reference Contig editor to analyze your assembly
Read more: Assembler: Using the coverage map of the Reference Contig editor to analyze your assemblyThere are two main steps to creating a reference assembly. Mapping your reads against your reference sequence and then analysing the alignment for variations. Knowing the depth of reads, or coverage, of an alignment is important for both of these stages. A low average depth of coverage means that you have less confidence in the…
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Choosing the default application to open a file type
Read more: Choosing the default application to open a file typeSometimes you’ll find that when you double click on a document (for example a protein sequence) that it opens in the wrong application. Generally this has resulted from recently installing a new application that has registered itself as the default application that you normally use to open that document. This will overwrite your default application.…
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Using old subsequence files with MacVector
Read more: Using old subsequence files with MacVectorSometimes when a subsequence file, that has been created by an version of MacVector prior to MacVector 11, has been stored on a remote filesystem or has been emailed MacVector will refuse to open the file. Mac files are generally comprised of two pieces: A data fork and the resource fork. Prior to the release…
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Problems with DMG appearing not to mount.
Read more: Problems with DMG appearing not to mount.Sometimes when you download the MacVector disk image and try to mount it nothing appears to happen. Generally this is because although it has been mounted a Finder window has not popped up. It also affects those of us with messy desktop (not me of course!). If you have many files then you might miss…
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MacVector 10.6 and earlier and Lion
Read more: MacVector 10.6 and earlier and LionIf you have upgraded to Lion and you’re running MacVector 10 to 10.6 (earlier versions will not run) then anytime MacVector displays an error message it will crash. MacVector 10.6 was developed before even Snow Leopard was released and is quite long in the tooth now. Why not take this opportunity to upgrade to MacVector…
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Designing primers that do not bind at multiple places in your template
Read more: Designing primers that do not bind at multiple places in your templateThe default settings for Primer3 do not actually check for whether a primer will bind at multiple places in the template. To turn this on use the PRIMER vs PRODUCT setting in the PRIMER BINDING tab (the Primer3 parameter is “PRIMER_MAX_TEMPLATE_MISPRIMING”). The default value is -1, which means do not check. Change this to 1…
