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Annotating the sequence in the Editor tab
Read more: Annotating the sequence in the Editor tabYou can very quickly annotate a region of interest in your sequence in the Editor tab. For example showing introns in lower case or highlighting CDS features with a colored background. To enter sequences as mixed case. To color a region. To change the case of a region.
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Removing a restriction site from a plasmid with the Cloning Clipboard
Read more: Removing a restriction site from a plasmid with the Cloning ClipboardBeing able to remove a restriction site by digesting with an enzyme that cuts at one site to linearize a plasmid, Klenow treating the linear fragment, then religating the fragment, is a useful trick in the lab. It can help you produce a suitable, and simple, cloning site when none previously existed in your vector.…
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Keep up to date with MacVector auxiliary files
Read more: Keep up to date with MacVector auxiliary filesMacVector has a large collection of data files that are installed in folders alongside the MacVector application. These include Restriction Enzymes files, Scoring Matrices, documentation, tutorials, a variety of common cloning vectors and even a few useful Applescripts. If you use MacVector’s built-in automatic updater to install each new release and bug-fix, you will find…
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Using BLAST to automatically annotate a sequence
Read more: Using BLAST to automatically annotate a sequenceYou can use the Database->Auto-Annotate Sequence function to quickly annotate a bare sequence using existing annotated sequences on your file system. However, this only works if your collection of sequences contains features representing all parts of the bare sequence. Luckily, if you have an unannotated region after running Auto-Annotate, you can use MacVector’s built-in BLAST…
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How to import annotated GenBank sequences via copy and paste
Read more: How to import annotated GenBank sequences via copy and pasteWhile MacVector does have a built-in Entrez browser (Database > Internet Entrez Search) you can easily import GenBank formatted text into MacVector via a simple copy and paste approach. Many sequence-oriented web sites have the option of viewing sequences in GenBank format. This format always starts with the text LOCUS and finishes with two forward…
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Troubleshooting problems with MacVector
Read more: Troubleshooting problems with MacVectorMacVector generally just works. However, it’s a very rare piece of software that does not have occasional technical faults. Every now and again we do get reports to MacVector Support about such technical issues. Whenever we come across such issues we document them and also try to fix it so they never happen again in…
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Visualising ORF analysis results in the MAP tab.
Read more: Visualising ORF analysis results in the MAP tab.The Map tab of MacVector is a powerful way to visualise and interact with your sequences. All analysis tools will work directly in the Map tab. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more. In fact one of the only tasks, you will need…
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MacVector 13.5.3 is out
Read more: MacVector 13.5.3 is outThere’s a new release of MacVector out. If you are on OS X 10.10 to OS X 10.7 then just update through Sparkle as normal. However, we have seen issues doing that on Snow Leopard, so please use the downloadable installer instead. Ironically some of the bug fixes are for OS X 10.6, but there’s…
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Keep Blast running on MacVector 12.7.5 and earlier versions.
Read more: Keep Blast running on MacVector 12.7.5 and earlier versions.If you are running MacVector 12.7.5 or earlier Blast will stop working for you from the end of this month. The fix for this is below. MacVector makes it easy to run a Blast search from your Desktop, and retrieve the hits directly back into MacVector. MacVector does this by submitting these Blast searches directly…
