How to quickly design primers to amplify a feature on your sequence

To design a pair of primers to amplify a single feature is pretty quick with MacVector.

  • Select a feature in the MAP tab
  • Run Primer Design (Primer3)
  • Ensure the dropdown menu is set to AMPLIFY FEATURE
  • Click OK
  • Check the summary shows that primers have been found and select the spreadsheet and graphical view
  • Click OK
  • Primer3

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