General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Importing sequences from Addgene into MacVector

The Addgene repository is a great place for obtaining expression vectors and other plasmids for your research. Fully annotated sequences are available for most plasmid sequences, although older plasmid deposits may be only partially annotated. All are very easy to import into MacVector.

All Addgene sequence deposits have a Genbank formatted sequence. This should contain the annotated sequence available for the plasmid entry. There is no advantage at all in downloading the sequence in any other format.

Just download the Genbank file and double click to open it in MacVector. MacVector will parse all annotation and present you with a full graphical map of your sequence. As long as the Genbank file is correct, this should be identical to the plasmid map on the web page.

You can also copy a sequence from a browser and go to FILE | NEW FROM CLIPBOARD.. to automatically import.

Addgene

Don’t forget that if you are using MacVector 15.5 or later then you will also see automatically annotated restriction enzymes and open reading frames.

Genbank is a fully open and universally supported sequence format and supports ALL biological annotation and features contained in a sequence. Genbank is the format used by the NCBI, EMBL and DDBJ. It’s updated yearly and ensures that any sequence is viewable by any researcher worldwide, regardless of the software they are using.

MacVector is fully committed to being open with sequence data and ensuring researchers can always exchange sequences without being locked into a proprietary format. We always fully support the import and export of Genbank sequences and we always update each new release of MacVector to be fully compatible with the Genbank format.

(August 2, 2017 edited with a few corrections from Addgene via Twitter! Thanks!).

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