General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

What can MacVector do for your lab?

Here’s an overview of what MacVector 15.5 can do for you!

Comparing sequences

Whatever alignment your sequence needs, MacVector has the right tool.

Cloning

CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target.

Multiple sequence alignment of protein or DNA sequences using CLustalW/Muscle or T-Coffee and produce publication quality alignments.

Sequence assembly of NGS data against a reference genome or sequencing of your new construct.

Search for similar sequences in the NCBI’s Genbank database with your sequence using BLAST or search your own local set of sequences.

Translated Alignments: Align DNA sequences based on their translations.

Align proteins against a reference comparing known proteins against unknown ones.

Characterizing sequences

Auto Annotation of sequences with common plasmid features.

InterProScan: Scan proteins for functional domains.

Cloning

Automatic ORF Display visualize open reading frames.

Graphical BLAST Results Visualization of your BLAST search..

MacVector

Design Cloning workflows

As simple as dragging a fragment to a cloning vector.

Flexible Cloning Subclone with restriction enzymes, Gibson cloning, Gateway and more.

Cloning history Every ligation in the construction of a plasmid is documented, wth enzymes used, any end modification, source of the fragment and date.

Agarose Gel to run out digested sequences. Easily identify restriction site(s) to screen successful clones from incorrectly ligated inserts.

Primer Design

MacVector makes primer design both easy AND powerful.

QuickTest Primer changes primer design. Hairpin? Nudge your primer until it goes.

Add tails to your primers with silent restriction sites/mismatches and view reading frame changes.

Quickly design pairs of primers click a region to get the best primer pairs to amplify it.

Primer Database store your primers and scans sequences for potential binding sites.

MacVector

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