General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

A whole new way to design primers with MacVector’s Quicktest Primer

QuickTest Primer completely changes the way primers can be designed on a computer. It simplifies primer design by showing your primer and its statistics in realtime. Does your primer have a hairpin? Nudge it along your template until the hairpin goes? Want to add a restriction site? Then add one and again nudge your primer to optimise the oligo. Want to see how a CDS translation is affected by your modified primer? View both the original and modified amino acid sequence. Nudge your primer base by base along the template until it goes. You can add tails to your primers with silent restriction sites/mismatches and view reading frame changes in frame.

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