MacVectorTip: How to find Restriction Enzymes that only cut outside of a specific region

One common cloning related task is to ask MacVector to find restriction enzyme sites that cut in a molecule, but that do not cut in a specific region. e.g. suppose you want to find restriction enzymes that cut pBR322 but that do not cut in the Tetracycline Resistance Gene. To do this, choose the Analyze | Restriction Enzyme... menu item and run a search using All Enzymes with your favorite .renz file (I used New England Biolabs.renz). When the search completes you’ll see a result dialog. Click on the With no cuts in checkbox, then click in the feature selection “note” icon to display a popup menu and select tetracycline resistance gene from the CDS sub-menu.

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You can then see in the Restriction Map result tab that none of the displayed enzymes cut in the Tet gene.

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