General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Category Archives: Tips

MacVector’s Scan For Missing Features tool makes beautiful plasmid maps easy!

Scan for.. Missing Features: Sequences are automatically scanned and missing features displayed. A simple right-click converts them to a permanent feature. Even blank sequences will be displayed fully annotated with common features. You can even add your own proprietary features. Tweet

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Testing pairs of primers with MacVector

MacVector’s Primer Design tools makes it easy to test your primers. You can insert them directly from MacVector’s Primer Database or copy and paste them. Tweet

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What can MacVector do for your lab?

Here’s an overview of what MacVector 15.5 can do for you! Comparing sequences Whatever alignment your sequence needs, MacVector has the right tool. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Multiple sequence alignment of protein or DNA sequences using CLustalW/Muscle or T-Coffee and produce publication quality alignments. Sequence assembly […]

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How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.

How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools. Tweet

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Downloading hits from the MacVector 15.5 BLAST Map results tab

The MacVector 15.5 BLAST Map results tab is a unique interface for examining the annotations around hits to a query sequence. Each pane in the display represents a High Scoring Segment Pair, as seen in the BLAST Aligned Sequence tab. At the lower left corner of each pane is a Download button – when you […]

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Controlling Automatic ORF Display

MacVector 15.5 automatically scans every DNA sequence window for open reading frames and displays the results in the Map tab. The setting for this are controlled by the MacVector | Preferences | DNA Map pane, along with the automatic Show restriction sites settings. The Minimum Number of Codons setting is fairly obvious. 5’ ends are […]

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Use the BLAST Map to better identify blast hits

With the advent of cheap Next Generation Sequencing technologies, there has been an explosion of whole genome sequences deposited in BLAST databases. One consequence of this is that, particularly for sequences of bacterial origin, most of the significant hits are to entire genomes. The classic BLAST results show the sequence alignments, but give no indication […]

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Generating a primer report to send to your Oligo Synthesis service

QuickTest Primer is a great tool for primer design. Paired with Primer Design/Test (Pairs) it gives you great control and flexibility for designing primers with tails, mismatches, silent mutations, one out sites and more. Once you’ve designed your primer the next step is to get it synthesized. QuickTest Primer will produce a PDF report of […]

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How to toggle between 1 and 3 letter amino acid codes

MacVector displays amino acid translations in many different result windows. You can drill down to the residue level in the Map tab and see translations of CDS and other translatable features and see translations in the plain text views and the Quicktest Primer interface. The translations can be viewed as either single letter codes or […]

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MacVector’s compatibility with different versions of macOS/OS X

We strive to ensure MacVector has good forwards and backwards compatibility. For example, MacVector 15.1 will still open files created with the very first version of MacVector! However, since Apple releases a new operating system every year, and generally make fairly significant “under the hood” changes, it is just not possible to support every version […]

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