Creating cloning construction flowcharts in third party applications

We’ve previously discussed how every ligation is documented. You get a “Frag” annotation that contains the date, source sequence, enzymes used and any end modification that was done to that fragment during the ligation.

However, we had regular requests to make it easier for user to document their constructs in other ways. For example being able to construct a flow diagram showing how a construct was made for your lab book.

So in MacVector 14.5 we added PDF export to the Cloning Clipboard. Now you can copy and paste a fragment as a PDF in a single step. The image can be pasted into any graphical application, such as Illustrator.

This makes it easy to create a graphical illustration of a cloning strategy. The fragment is copied, exactly as it is displayed in the Cloning Clipboard showing restriction enzymes that have been used to digest it.

  • Select the fragment in the Cloning Clipboard.
  • Use EDIT > COPY
  • Move to your application and use EDIT > PASTE
  • All fragments are copied as linear images, so if you need to show a circular vector, then in the Map view just copy and paste that.

    In this first example here’s a document (in Omnigraffle) showing a simple ligation.


    The fragments are copied at a fixed width size, irrespective of the actual fragment sequence length. However, if you need finer control over your fragment before creating a PDF of it, then simply double click on the fragment. It will appear in a new MacVector sequence window which you can modify before copying it as a PDF.

    In the second document here’s three fragments all inserted into a single Gateway vector via Multisite Gateway cloning.


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