General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Using the Primer Database to store your lab’s collection of primers

MacVector’s Primer Database allows you to save and retrieve primers from a Primer Database with the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites using Primer Database Search. The tool comes with a starter database of primers, but you can use existing subsequence files or import primers from Excel.

NewImage

To save a primer from QT Primer:

  • Open ANALYZE > QUICKTEST PRIMER (INDIVIDUAL)
  • Design your primer
  • Click ADD TO…
  • Give the primer a name and add a comment. Click OK
  • To save a primer from Primer Design:

  • Open ANALYZE > PRIMER DESIGN/TEST (PAIRS)
  • Design your primer pair
  • In the spreadsheet right click on a primer
  • Choose ADD PRIMER TO DATABASE
  • Give the primer a name and add a comment. Click OK
  • To use the Primer Database Search:

  • Open your sequence
  • Select ANALYZE > PRIMER DATABASE SEARCH
  • Choose parameters and click OK
  • This entry was posted in Uncategorized and tagged , , . Bookmark the permalink. Trackbacks are closed, but you can post a comment.

    Post a Comment

    Your email is never published nor shared. Required fields are marked *

    You may use these HTML tags and attributes <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <s> <strike> <strong>

    *
    *