General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Confirming a small sequencing project against a reference sequence

Align to reference is a perfect tool for mapping small sequencing projects against a reference sequence. It’s perfect for accurately and quickly:
– Confirming the sequence of a cloned fragment
– Sequencing across the ends of a cloned fragment to confirm the junction sequence
– Screening clones from a site-specific mutagenesis experiment to identify successful mutations
– Screening related clones for single nucleotide polymorphisms

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