Designing a pair of primers to amplify a single feature is pretty quick with MacVector. Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer, which means any custom ends (with mismatches or new restriction sites) are also included.
Annotating primers to your sequence with MacVector
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