General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Category Archives: Tutorials

Use Database | Auto-Annotate Sequence to annotate prokaryotic genomes

The continuing advances in Next Generation Sequencing have made it relatively low cost to sequence prokaryotic genomes. Many scientists are embarking on large projects to sequence multiple related genomes. These might be clinical isolates of the same species exhibiting different pathogenetic properties, environmental isolates from different sites, or a study over time of the changes […]

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Using MacVector’s Auto Annotate tool to annotate blank sequences.

  Have you ever been sent a plain unannotated sequence, or downloaded a sequence from Entrez and been disappointed as it doesn’t have the carefully curated graphical appearance of your favorite genes? Auto annotation solves both of these common problems. The basic idea is that you can scan the sequence against a folder containing a […]

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Adding a primer to MacVector’s Primer Database.

  MacVector’s Primer Database tool makes it easy to store your lab’s regular primers. It allows you to save and retrieve primers from the Primer Database from the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites. The Primer Database will also store tails and allow you to specify […]

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RNASeq Expression Analysis with NGS data

If you have the Assembler module, MacVector can align millions of NGS reads from RNASeq experiments against large genomes and generate a coverage table displaying the relative expression levels of every gene in a genome. The key to this functionality is that you must have a reference genome with genes annotated as CDS or gene […]

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A whole new way to design primers with MacVector’s Quicktest Primer

QuickTest Primer completely changes the way primers can be designed on a computer. It simplifies primer design by showing your primer and its statistics in realtime. Does your primer have a hairpin? Nudge it along your template until the hairpin goes? Want to add a restriction site? Then add one and again nudge your primer […]

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Designing and documenting cloning strategies

Designing cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. However, when you open this construct a year later, you don’t want to have to look back in your lab book to know how you generated it. Neither do you need to! MacVector fully […]

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MacVector 13 training Workshop at the Gurdon Institute

When: Thursday 23rd October from 11:30 until 13:30 Where: The Gurdon Institute, Level 1 meeting room Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last few years. […]

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Importing sequences from ENSEMBL

There’s a few different ways to import annotation from the ENSEMBL database browser, as well as other databases. Using Genbank The easiest way to export from ENSEMBL and keep all annotation is to use the Genbank format. The default format will be FASTA which has no annotation. With Genbank all the annotation is stored in […]

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MacVector 12.7 Training Workshop, Weatherall Institute for Molecular Medicine

When: Tuesday, 23rd of April 2013, 14:00 until 16:00 Where: WIMM Seminar Room, WIMM Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last two years to MacVector. […]

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MacVector 12.7 Training Workshop at Emory University

When: Monday March 25, 1:00-3:00 pm Where: Calhoun Auditorium, Clinic B building “Tunnel Level” Dr. Kevin Kendall of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last 2 years to […]

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