Month: April 2015
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How to quickly design primers to amplify a feature on your sequence
To design a pair of primers to amplify a single feature is pretty quick with MacVector.
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Quickly checking a small sequencing project
For analysing large sequencing datasets, whether de novo or mapping reads against a reference you need Assembler. However, many times you do not need a powerful tool but just a quick way to check some sequencing data. For example for checking small sequencing projects, such as a site directed mutagenesis, looking for SNPs in a…
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101 things you (maybe) didn’t know about MacVector: #42 – Managing segmented features
If you download eukaryotic genomes from GenBank, you will find that many coding (i.e. CDS) features are segmented and consist of multiple individual segments joined together into a single long feature. You can see an example of this with the human cystic fibrosis transmembrane regulator gene – you find a copy of this in the…