Month: June 2024
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MacVectorTip: Quality scoring of manual edits to your contigs.
Quality scoring of Assemblies and Align to Reference alignments can be visualized directly on the sequence. Residues can be shaded according to their quality scores. These can be displayed anywhere quality values are available, including de novo and reference assemblies in Assembler and Align to Reference alignments. The intensity of the shading of residues indicates…
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How to design a digest to screen minipreps after a ligation.
MacVector’s Agarose Gel tool can be used to quickly design a restriction digest to screen minipreps following a ligation. Replicate your ligation in MacVector. Create your agarose gel with the correct insert and a vector only lane. Undo the ligation, and repeat with the wrong orientation. Now you will end up with an Agarose Gel…