Author: Chris
-
Searching and downloading sequences from Entrez
MacVector has integrated connectivity to the NCBI BLAST and Entrez databases. You can directly search Entrez for DNA or Protein sequences based on features, authors, keywords etc and directly download them into MacVector, complete with all features and annotations.
-
Eastern Great Lakes workshop tour in February
The MacVector team will be touring the Eastern Great Lakes for a series of workshops in February. We will be running workshops in Rochester NY, Buffalo NY, Ypsilanti MI, Cleveland OH, Wooster OH, Columbus OH and Cincinnati OH. Monday, Feb. 5th 10:00 – 12:00 University of Rochester, Rochester, NY 2:00 – 4:00 University at Buffalo,…
-
Using MacVector’s Auto Annotate tool to annotate blank sequences.
Have you ever been sent a plain unannotated sequence, or downloaded a sequence from Entrez and been disappointed as it doesn’t have the carefully curated graphical appearance of your favorite genes? Auto annotation solves both of these common problems. The basic idea is that you can scan the sequence against a folder containing a…
-
Adding a primer to MacVector’s Primer Database.
MacVector’s Primer Database tool makes it easy to store your lab’s regular primers. It allows you to save and retrieve primers from the Primer Database from the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites. The Primer Database will also store tails and allow you to specify…
-
RNASeq Expression Analysis with NGS data
If you have the Assembler module, MacVector can align millions of NGS reads from RNASeq experiments against large genomes and generate a coverage table displaying the relative expression levels of every gene in a genome. The key to this functionality is that you must have a reference genome with genes annotated as CDS or gene…
-
Annotating primers to your sequence with MacVector
Designing a pair of primers to amplify a single feature is pretty quick with MacVector. Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer,…
-
Use a right-click in the Contig Editor tab to see if your contig can be circularized
MacVector 16 incorporates no less than THREE different de novo assemblers, phrap, velvet and SPAdes. While all are great assemblers, with each having their own specific advantages, none of them will generate a circular sequence from input reads. However, MacVector 16 also includes a new feature to help you with this. If you are assembling…
-
MacVector are at the ASCB – EMBO 2017 meeting in Philadelphia
The MacVector team will be at the ASCB/EMBO 2017 meeting this coming Sunday. The conference starts on Sunday the 3rd of December and runs until Tuesday the 5th. It’s being held at the Pennsylvania Convention Center. Our booth is 1002. If you are in Philadelphia for the conference please pop along and say “hi!”. We…
-
Downloading hits from the MacVector BLAST Map results tab
MacVector’s BLAST Map results tab (added in MacVector 15.5) is a unique interface for examining the annotations around hits to a query sequence. Each pane in the display represents a High Scoring Segment Pair, as seen in the BLAST Aligned Sequence tab. At the lower left corner of each pane is a Download button –…
-
Turning on/off the SCAN FOR missing Features and ORFs
If you’re running MacVector 15.5 or later then you will have noticed extra features annotated to your sequences. These are from the Scan for ORFs tool (added in MacVector 15.5) and the Scan For Missing Features (added in MacVector 16) tools that automatically scan every DNA sequence window for open reading frames, missing annotations and…