Visualizing ORF analysis results in the MAP tab

The Map tab is a powerful way to visualize and interact with your sequences. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more.

The Map tab of a Results window is just as flexible as the Map tab of the main sequence window. You can Zoom to Residue to see your sequence results at the sequence level, or Drag to Zoom to show just a small region.

A useful way to visualize an ORF analysis is via the Map tab, except zoomed to the sequence level, rather than using it as a graphical overview. When you initially run ANALYZE > OPEN READING FRAMES.. the Map tab in the results window shows the entire sequence, with graphical symbols representing ORFs. However, if you click ZOOM TO RESIDUE in the Graphics Palette, then instead you will see your sequence, any annotated features, and the amino acid sequence of any ORFs in your sequence.

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You can also directly analyze the results within the Map tab. Just click on an ORF and copy that sequence to a new one, or do a Blast search directly on that sequence to see if it’s the coding region you are looking for.

  • Run ANALYZE > OPEN READING FRAMES…
  • Choose the appropriate settings and click OK
  • Switch to the Map tab in the Results window
  • Click Zoom to Residue in the Graphics Palette
  • Select the appropriate ORF
  • You can either copy it and use FILE > NEW FROM CLIPBOARD to create a new sequence, or DATABASE > BLAST to search for it.
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