Tag: codon optimization

  • MacVector 18.7 has just been released.

    MacVector 18.7 has just been released.

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    by

    Chris
    in

    MacVector 18.7 has just been released. If you are eligible for this release you will be prompted to upgrade, otherwise go to MACVECTOR | CHECK FOR UPDATES… and follow the prompts to be automatically upgraded. If your license is not eligible then why not upgrade? Overview MacVector 18.7 introduces a History tab to track the…

  • MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translation

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    Chris
    in

    MacVector uses codon usage tables (also called “Codon Bias”) to help you predict protein coding regions in the Analyze | Nucleic Acid Analysis Toolbox functions and also the protein Analyze | Reverse Translation… function. For example, here are the Plus and Minus strand Staden Codon Preference plots aligned under the Plus/Minus ORFs for a Streptomyces…

  • How to find one-out Restriction Sites that will change a protein coding sequence

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    by

    Chris
    in

    If you are interested in making changes to a protein sequence, it is often useful to make a change to the coding DNA that will create a restriction enzyme site. Conversely, there may be times that you would like to create a site without affecting a protein coding region. You can accomplish this using the…

  • Optimizing the Reverse Translation function

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    by

    Chris
    in

    The Analyze | Reverse Translation menu option lets you create a DNA sequence from a Protein sequence, reverse translated using a specific Genetic Code (by default, the Universal Genetic Code). The default option creates a DNA sequence with N’s and other ambiguities reflecting the degeneracy of the genetic code. This is great if you want…