Tag: primer
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MacVectorTip: Use Quicktest Primer to design primers that change encoded amino acids and create restriction enzyme sites
The Quicktest Primer interface is highly interactive and the display shows restriction enzymes and the amino acids sequences of CDS features in the region of interest. Here’s a ~25nt primer aligned against a parental sequence. Restriction enzyme recognition sites are shown in black text. “One-out” sites (e.g. a 5 out of 6 match) are shown with an…