Month: January 2017

  • Functional domain analysis of protein sequences using InterProScan

    —

    by

    Chris
    in ,

    There’s a new tool in MacVector 15 that allows you to do functional domain analysis on your protein sequence using the InterProScan service. InterPro contains multiple databases of protein families, domains and motifs and InterProScan will submit a protein sequence to a search of these databases. It will also do extra analysis such as transmembrane…

  • Create your own Primer Database.nsub from an Excel spreadsheet

    —

    by

    Chris
    in ,

    Wouldn’t it be great if there was an easy way of converting that huge primer collection you have into a format that MacVector can use? Well, luckily there is! There is a utility called “Primer Converter” that you can download from our website. To use the utility you first need to get your primers into…

  • Use Analyze->Primer Database Search to scan sequences for primer binding sites

    —

    by

    Chris
    in

    It’s easy to keep track of all your primers using a MacVector subsequence (.nsub) file. We even ship MacVector with a starter file called “Primer Database.nsub” that you can find in the /Applications/MacVector/Subsequences/ folder. If you choose Analyze | Primer Database Search… and use this default file to search pUC19, you’ll find many of the…

  • Batch auto annotation of blank sequences with MacVector and AppleScript

    —

    by

    Chris
    in

    Over the past few releases we’ve been making more MacVector tools to be scriptable with AppleScript. The latest is Auto Annotation. Auto Annotation is a great tool for curating your sequences. For example if you receive a unannotated sequence then you can scan it against other sequences to find. Blasting an unknown sequence, fetching the…

  • MacVector For Windows update

    —

    by

    Chris
    in

    It’s been some time since since the last update about MacVector for Windows, although development has certainly not been quiet. Here’s some screenshots. Graphical Map Sequence Editor Automatic Restriction Enzyme and ORF Analysis Primer Design Cloning Clipboard

  • Using the Primer Database to store your lab’s collection of primers

    —

    by

    Chris
    in

    MacVector’s Primer Database allows you to save and retrieve primers from a Primer Database with the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites using Primer Database Search. The tool comes with a starter database of primers, but you can use existing subsequence files or import primers from…

  • Viewing external database entries for features in a sequence.

    —

    by

    Chris
    in

    Sequences, or regions of sequences, can be linked to external databases. For example an entire sequence entry or for when annotation tools are used to annotate proteins with domain or motif information (e.g. InterProScan available in MacVector 15). Very useful for when you want to view more detailed or updated information. Within the Genbank specification,…