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How to quickly design primers to amplify a feature on your sequence
Apr 23, 2015
—
by
Chris
in
Tips
To design a pair of primers to amplify a single feature is pretty quick with MacVector.
Select a feature in the MAP tab
Run Primer Design (Primer3)
Ensure the dropdown menu is set to AMPLIFY FEATURE
Click OK
Check the summary shows that primers have been found and select the spreadsheet and graphical view
Click OK
pcr
primer3
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