-
Workflows on designing, testing and storing primers in MacVector
Read more: Workflows on designing, testing and storing primers in MacVectorMacVector is the best PCR Primer design application on macOS. It has many innovative primer tools to make designing, analyzing and cataloging your primers or oligos easy. Here are a few typical workflows. Designing primers Amplifying a gene You can design a set of primers to amplify a gene in as little as three mouse…
-
MacVector 16 and macOS High Sierra 10.13.4.
Read more: MacVector 16 and macOS High Sierra 10.13.4.Apple have just released macOS® High Sierra 10.13.4. This may appear to be just an incremental update to macOS High Sierra. However, under the hood there are some major changes, not least is a new warning dialog if you are running an older application on macOS High Sierra. With the release of macOS High Sierra 10.13.4…
-
Assemble bacterial genomes in minutes on your Mac laptop
Read more: Assemble bacterial genomes in minutes on your Mac laptopMacVector with Assembler contains some remarkably powerful algorithms for assembling Next Generation Sequencing (NGS) data. Not so long ago, you needed a powerful Linux server with lots of memory for de novo assembly of whole genomes. But with advances in the efficiency of algorithms and improvements in hardware, it is now possible to assemble quite…
-
Simple Assembly of Sanger Sequencing Files with MacVector Assembler
Read more: Simple Assembly of Sanger Sequencing Files with MacVector AssemblerWith MacVector Assembler, assembling ABI Sanger Sequencing files is simple, fast and accurate. MacVector uses the popular phred/phrap/cross_match set of tools from the University of Washington. To improve accuracy, and to help resolve repeats, these tools use “quality scores” (popularly known as “phred scores”), giving them an advantage over many other methods. To assemble two…
-
Assembling sequencing data with MacVector and Assembler
Read more: Assembling sequencing data with MacVector and AssemblerMacVector has a software plugin called Assembler that integrates directly into the DNA sequence analysis toolkit and provides DNA sequence assembly functionality. Dealing with sequencing reads has never been easier. MacVector includes no less than five different assemblers just a few mouse clicks away from your sequencing reads. Phrap assembles Sanger sequencing reads or existing…
-
MacVector video tutorials on You Tube
Read more: MacVector video tutorials on You TubeThere are short video tips on using MacVector on our blog and our YouTube channel. Each one is less than 2 minutes and generally shorter than that! There will be a new screencast every few weeks, so please subscribe to our YouTube channel so you don’t miss any! The latest screencasts include quickly annotating a…
-
Viewing external database entries for features in a sequence.
Read more: Viewing external database entries for features in a sequence.Sequences, or regions of sequences, can be linked to external databases. For example an entire sequence entry or for when annotation tools are used to annotate proteins with domain or motif information (for example InterProScan). Very useful for when you want to view more detailed or updated information. Within the Genbank specification, which MacVector extensively uses,…
-
Use the Replica Button For Synchronized Views
Read more: Use the Replica Button For Synchronized ViewsMost primary MacVector windows (Nucleic Acid Sequence, Protein Sequence, Multiple Sequence Alignment, Align To Reference, Contig Assembly etc.) have a Replica toolbar button. If you click that button, a second window will open, potentially set to a different tab. The key to this functionality is that the two windows are linked – any selections you…
-
How to Identify Bacterial Promoters Using MacVector
Read more: How to Identify Bacterial Promoters Using MacVectorMacVector’s Subsequence tool is a very flexible search function that can be used for a variety of tasks. MacVector itself has a built-in variant of the function for maintaining and search primer databases (Analyze | Primer Database Search…). Each entry in the file MacVector uses as a source of subsequence data can have up to…
-
Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features
Read more: Import Multi-Sequence Genbank Files into an Assembly Project for easy access to FeaturesThere are many genomes in the Genbank database that cannot be downloaded as single annotated sequences. These might be large multi-chromosome eukaryotic genomes, but, increasingly, partially sequenced bacterial chromosomes where the major contigs have been annotated using the NCBI annotation pipeline. Typically, when you encounter these, there are options to download annotated versions of these…
