MacVector’s Primer Database allows you to save and retrieve primers from a Primer Database with the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites using Primer Database Search. The tool comes with a starter database of primers, but you can use existing subsequence files or import primers from Excel.
To save a primer from QT Primer:
- Open ANALYZE > QUICKTEST PRIMER (INDIVIDUAL)
- Design your primer
- Click ADD TO…
- Give the primer a name and add a comment. Click OK
To save a primer from Primer Design:
- Open ANALYZE > PRIMER DESIGN/TEST (PAIRS)
- Design your primer pair
- In the spreadsheet right click on a primer
- Choose ADD PRIMER TO DATABASE
- Give the primer a name and add a comment. Click OK
To use the Primer Database Search:
- Open your sequence
- Select ANALYZE > PRIMER DATABASE SEARCH
- Choose parameters and click OK