MacVector’s Primer Database allows you to save and retrieve primers from a Primer Database with the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites using Primer Database Search. The tool comes with a starter database of primers, but you can use existing subsequence files or import primers from Excel.
To save a primer from QT Primer:
Open ANALYZE > QUICKTEST PRIMER (INDIVIDUAL)
Design your primer
Click ADD TO…
Give the primer a name and add a comment. Click OK
To save a primer from Primer Design:
Open ANALYZE > PRIMER DESIGN/TEST (PAIRS)
Design your primer pair
In the spreadsheet right click on a primer
Choose ADD PRIMER TO DATABASE
Give the primer a name and add a comment. Click OK
To use the Primer Database Search:
Open your sequence
Select ANALYZE > PRIMER DATABASE SEARCH
Choose parameters and click OK
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