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Smart Folders and MacVector
Read more: Smart Folders and MacVectorOS X’s Finder has many features for quickly finding and working with your files. Spotlight Search is one such tool that most Mac users are familiar with. However, Smart Folders is a tool that is very useful but often overlooked. Smart Folders allow you to create a dynamic folder whose contents are derived from a…
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101 things you (maybe) didn’t know about MacVector: #44 – Opening matching sequences from an Align To Folder search
Read more: 101 things you (maybe) didn’t know about MacVector: #44 – Opening matching sequences from an Align To Folder searchThe Database | Align To Folder function is an extremely useful tool to help you find matching sequences on your own local file system. It is essentially a BLAST search of your own private sequence collection – a little slower, but more sensitive. You can use it to easily open all of the sequences you…
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MacVector at ASM2015
Read more: MacVector at ASM2015We’ll be at the 115th General Meeting of the American Society for Microbiology from the 30th of May to the 2nd of June in New Orleans. We’re on booth 366. Please do drop by and say hello. We’ll be able to show you the upcoming release of MacVector 14 and other cool stuff. We’d also…
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101 things you (maybe) didn’t know about MacVector: #43 – Compatible Restriction Enzyme sites have special highlighting
Read more: 101 things you (maybe) didn’t know about MacVector: #43 – Compatible Restriction Enzyme sites have special highlightingHave you ever wondered how you are going to clone a particular fragment into a specific vector? What destination restriction sites are compatible with the enzymes you’ve chosen? MacVector has a unique color-coding approach to make it easy to identify compatible sites. Here’s how it works; First, select the source fragment you wish to clone.…
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How to quickly design primers to amplify a feature on your sequence
Read more: How to quickly design primers to amplify a feature on your sequenceTo design a pair of primers to amplify a single feature is pretty quick with MacVector. Select a feature in the MAP tab Run Primer Design (Primer3) Ensure the dropdown menu is set to AMPLIFY FEATURE Click OK Check the summary shows that primers have been found and select the spreadsheet and graphical view Click…
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Quickly checking a small sequencing project
Read more: Quickly checking a small sequencing projectFor analysing large sequencing datasets, whether de novo or mapping reads against a reference you need Assembler. However, many times you do not need a powerful tool but just a quick way to check some sequencing data. For example for checking small sequencing projects, such as a site directed mutagenesis, looking for SNPs in a…
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101 things you (maybe) didn’t know about MacVector: #42 – Managing segmented features
Read more: 101 things you (maybe) didn’t know about MacVector: #42 – Managing segmented featuresIf you download eukaryotic genomes from GenBank, you will find that many coding (i.e. CDS) features are segmented and consist of multiple individual segments joined together into a single long feature. You can see an example of this with the human cystic fibrosis transmembrane regulator gene – you find a copy of this in the…
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Upgrade MacVector with a 30% discount during April
Read more: Upgrade MacVector with a 30% discount during AprilDon’t be an April Fool, upgrade with a…. 30% discount … before MacVector 14 is released in May. This is no April Fool’s joke. Here’s a great way to upgrade your copy of MacVector before the release of MacVector 14 next month. For the entire month of April you will get a 30% discount on…
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101 things you (maybe) didn’t know about MacVector: #41 – Extracting raw data from chromatogram files
Read more: 101 things you (maybe) didn’t know about MacVector: #41 – Extracting raw data from chromatogram filesHave you ever wanted to know exactly what the total signal value is for individual peaks in a chromatogram file? Perhaps you are looking for mixtures of residues at a particular location and want to get some idea of the relative proportions? You can open .ab1 and .scf chromatogram files directly in MacVector and view…
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UNIX line endings for plain text files
Read more: UNIX line endings for plain text filesSometimes you may need to export data out of MacVector for further analysis with a command line tool or sending a file to a colleague who does not have MacVector). For example exporting the VCF report out of a reference assembly or exporting a sequence into Genbank format. If these files are plain text, then…
