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MacVector at the ASM in Boston
Read more: MacVector at the ASM in BostonWe’ll be at the 114th American Society for Microbiology this coming Saturday, May 17-20, 2014 in Boston. Come visit us if you are also in Boston. We’re on booth 345. We’ll have demo CDs of our latest release, MacVector 13, and there’ll be mouse pads and incubator tube floaties to pick up. Probably candy too!…
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MacVector 13.0.1 released.
Read more: MacVector 13.0.1 released.We’ve just released a minor update, MacVector 13.0.1. This release has a few minor bug fixes that have been reported in the past month. However, this release also sees the introduction of online updating via Sparkle. This allows you to update your copy of MacVector in place. Once a day MacVector will check our website…
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MacVector at the NIH Research Festival April 23 – 24
Read more: MacVector at the NIH Research Festival April 23 – 24For all of our NIH users: We will be at booth # 314 at the NIH Research Festival that is Wed. – Thurs., April 23 – 24. Please stop by for a demo of our new release, MacVector 13, and some other freebies, including discounted Easter candy!
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Accessing BAM files from an Assembly Project file
Read more: Accessing BAM files from an Assembly Project fileAll assemblies are stored using the BAM file format. This is a binary file that stores each read and where and which consensus/contig/reference it is mapped against. It is a compressed version of the pure text SAM format. For some post assembly tasks it is necessary to do further processing on the BAM file. To…
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de novo assembly with Velvet
Read more: de novo assembly with VelvetVelvet is a short read aligner that works very well on a wide variety of reads. Velvet excels at de novo assembly of sequencing reads from second and newer generation sequencers. In our latest release, MacVector 13, we’ve added Velvet to Assembler. This joins the existing tools, Phrap for Sanger sequencing reads and Bowtie for…
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Calculating the melting temperature of PCR primers
Read more: Calculating the melting temperature of PCR primersCalculating an accurate melting temperature of your oligos, your template and of the predicted product is important to set the cycling parameters of your PCR machine. The Tm calculations in MacVector were updated in MacVector 12.6 to use a more modern algorithm. MacVector has always used thermodynamic “nearest neighbor” calculations, but there were two changes…
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Troubleshooting: Resetting MacVector’s preferences
Read more: Troubleshooting: Resetting MacVector’s preferencesMacVector generally just works. However, even the most well behaved of applications sometime have problems. If you have restarted the application and restarted your Mac then a good troubleshooting step to perform next is to reset your preferences. For Mountain Lion and earlier deleting the preferences meant opening up Finder, navigating to your ~/Library folder…
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Importing sequences from ENSEMBL
Read more: Importing sequences from ENSEMBLThere’s a few different ways to import annotation from the ENSEMBL database browser, as well as other databases. Using Genbank The easiest way to export from ENSEMBL and keep all annotation is to use the Genbank format. The default format will be FASTA which has no annotation. With Genbank all the annotation is stored in…
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Implementing a new activation code for network license users
Read more: Implementing a new activation code for network license usersWhen you’ve got a lot of licenses and a lot of computers to manage, a network license is the most effective way of letting your users access their favourite sequence analysis app for the Mac! MacVector network license use the KeyServer network license software from Sassafras. However, in addition to the KeyServer setup MacVector also…
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Testing primers with MacVector 13
Read more: Testing primers with MacVector 13New to MacVector 13 is the ability to quickly test a pair of primers. Previously to test a pair of primers with the Primer3 tool you needed to modify the expected product and reduce the stringency of all parameters so that your primers would be accepted. Now when you enter a pair of primers into…
