Tag: agarosegels
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How to design a digest to screen minipreps after a ligation.
MacVector’s Agarose Gel tool can be used to quickly design a restriction digest to screen minipreps following a ligation. Replicate your ligation in MacVector. Select the two sites, for subcloning your targeted gene, and click DIGEST. Drag the digested fragment from the Cloning Clipboard to your vector click LIGATE. Create your agarose gel with the…
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Simulating DNA electrophoresis in agarose gels using MacVector’s Agarose Gel tool
MacVector has a Agarose Gel interface which allows you to view photo-realistic recreations of restriction digests of linear and circular DNA molecules. The gels look so realistic that users have had a hard time telling photos of their own digests from the simulation in MacVector. When you first use the new tool and compare it…
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MacVectorTip: Simulating mixed plasmid populations in agarose gels
We had a recent support call this week from somebody who believed from their agarose gels that they had a mixed population of plasmids from an experiment and wanted to document and determine the banding pattern using MacVector’s agarose gel simulation. You might come across this type of scenario if you have been making site-specific…
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Using MacVector’s Agarose Gel tool to design a digest to screen minipreps after a ligation.
How to design a digest to screen minipreps after a ligation. (View full size on website…)
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What can MacVector do for me?
Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Sequence assembly of NGS data against a reference genome or compare your sequencing against your new construct. Translated Multiple Sequence…
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How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.
How to check the orientation of a ligated insert using MacVector’s Restriction Digest and Agarose Gel tools.
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Print Agarose Gel images with black bands on a white background
The new simulated agarose gel function in MacVector 14.5 creates photorealistic agarose gels that look just like the real thing. However, if you want to print a record of a gel on a laser printer, the standard white bands on a grey background are not always ideal, if for no other reason than it uses…
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Controlling the realism of Agarose Gels
The default settings for the agarose gel display in MacVector generate a reasonable photorealistic simulation of an agarose gel. We have actually boosted the intensity of small bands quite significantly, and reduced their diffusion so that smaller bands show up a little more obviously and crisply than they might in real life. Even so, you…