MacVector 26 sees the addition of Golden Gate Cloning to MacVector’s Cloning Project Manager
We are just putting the finishing touches to a new Golden Gate Cloning interface for our next release of MacVector. Based on our existing Gibson Assembly interface, this new functionality lets you design constructs using the popular Golden Gate approach. This takes advantage of Type IIS restriction enzymes like Bsa I and Bsm BI that cleave to one side of their recognition sequences leaving four nucleotide single stranded non-palindromic overhangs that enable the directional assembly of multiple fragments in a single reaction.
The new MacVector interface lets you use existing vectors or fragments with Type IIS sites alongside PCR primers designed to generate compatible 4nt overhangs when cleaved with the appropriate enzyme. The predicted constructs retain all of the standard nucleotide features allowing you to customize fragment junctions to the residue level for in-frame fusions.
If you are actively using Golden Gate cloning in your research and would like access to a preview version of the new functionality, please feel free to contact us at MacVector Support and we will be happy to send you a link to a beta version.