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Cloning Clipboard: A few tips on working with digested fragments
Read more: Cloning Clipboard: A few tips on working with digested fragmentsThe Cloning Clipboard is an easy, and flexible, way to design and document your cloning strategies. Recently we discussed how every ligation in a cloning procedure is documented and stored in a sequence, so you always know how a construct was made. If you need to manipulate a fragment as a sequence, before ligating it,…
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101 things you (maybe) didn’t know about MacVector: #48 – Setting the Alignment match/mismatch characters
Read more: 101 things you (maybe) didn’t know about MacVector: #48 – Setting the Alignment match/mismatch charactersThere are many output windows throughout MacVector that display aligned sequences. If you run an Align To Folder, Create Dotplot or Internet Blast Search, one or more of the output windows will show alignments in a plain text format with some sort of character indicating matching residues. The default is to use the vertical |…
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How Assembler uses quality scores to create assemblies
Read more: How Assembler uses quality scores to create assembliesA common problem with all types of sequence assembly is distinguishing between sequencing errors and true genomic variations. Quality scores are one way to help the algorithm identify if a variation is of high quality and therefore likely to be a SNP or a sequencing error. For Assembler trace files can be basecalled with Phred,…
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Visualising ORF analysis results in the MAP tab.
Read more: Visualising ORF analysis results in the MAP tab.The Map tab of MacVector is a powerful way to visualise and interact with your sequences. All analysis tools will work directly in the Map tab. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more. In fact one of the only tasks, you will need…
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Cloning Clipboard: Documenting the history of a construct
Read more: Cloning Clipboard: Documenting the history of a constructDesigning and documenting cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. Not only that but every step is documented and recorded in the resulting sequence, so you always know where each fragment came from. Ligating a single fragment into a vector is as…
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101 things you (maybe) didn’t know about MacVector: #47 – Optimal PCR Annealing Temperature
Read more: 101 things you (maybe) didn’t know about MacVector: #47 – Optimal PCR Annealing TemperatureI wrote about how MacVector calculates the melting temperature of a primer in an earlier blog post. One other common question we get is, “once I have designed a pair of primers, what is the optimum annealing temperature to use in the PCR?” MacVector calculates this and displays the results in the Analyze | Primer…
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MacVector 14.0.4 is OS X 10.11 El Capitan compatible.
Read more: MacVector 14.0.4 is OS X 10.11 El Capitan compatible.OS X 10.11 El Capitan was released last week. We’ve been testing MacVector on the developer releases of OS X EL Capitan in the run up to the official release. However, as usual we prefer to fully test on an official release of OS X before saying that MacVector is supported. We’ve now done so…
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Buy two copies of MacVector 14 for the price of one.
Read more: Buy two copies of MacVector 14 for the price of one.For the month of October if you buy a personal or standard license of MacVector Pro then you’ll get one free! We never stop working to keep MacVector your best solution for designing primers, subcloning into vectors, aligning your sequences and checking small sequencing projects on the Mac. So don’t miss this chance to start…
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Using the Primer Database to store your lab’s collection of primers
Read more: Using the Primer Database to store your lab’s collection of primersMacVector now directly supports storing primers within a primer database. The Primer Database tool allows you to save and retrieve primers from subsequence files within Primer3 and Quicktest Primer. You can easily create your own primer database, use existing files or import primers from Excel. Many users use subsequence files to store primers, and in…
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101 things you (maybe) didn’t know about MacVector: #46 – How to increase the number of graphics layers in the Map tab
Read more: 101 things you (maybe) didn’t know about MacVector: #46 – How to increase the number of graphics layers in the Map tabThe graphical maps of heavily annotated sequences can get busy in a hurry. You may end up with so many features overlapping a specific location on a sequence that the graphical images for those features “pile up” on top of one another at the top or bottom of the display. While this can happen with…
