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101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson Assemblies
Read more: 101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson AssembliesWe’ve had a few MacVector users ask us recently if MacVector can be used to create constructs using the increasingly popular “Gibson Assembly” method. For those who are not familiar with this method, it was first described in 2009 by Daniel Gibson of the J. Craig Venter Institute. They showed that you can assemble multiple…
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MacVector 12.7 Training Workshop, Weatherall Institute for Molecular Medicine
Read more: MacVector 12.7 Training Workshop, Weatherall Institute for Molecular MedicineWhen: Tuesday, 23rd of April 2013, 14:00 until 16:00 Where: WIMM Seminar Room, WIMM Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last two years to MacVector.…
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MacVector 12.7 Training Workshop at Emory University
Read more: MacVector 12.7 Training Workshop at Emory UniversityWhen: Monday March 25, 1:00-3:00 pm Where: Calhoun Auditorium, Clinic B building “Tunnel Level” Dr. Kevin Kendall of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last 2 years to…
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Using Blast and Entrez in MacVector with a proxy server
Read more: Using Blast and Entrez in MacVector with a proxy serverBlast and Entrez connect to the NCBI server using the normal “http” ports (exactly the same as if it was a web browser). If a web browser can access the NCBI’s server then MacVector should be able to. The address for the Blast server is: http://www.ncbi.nlm.nih.gov:80/blast/Blast.cgi However, if you get the following error message then…
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101 things you (maybe) didn’t know about MacVector: #26 – Creating Features Automatically
Read more: 101 things you (maybe) didn’t know about MacVector: #26 – Creating Features AutomaticallyFollowing on from my recent posts on manual and semi-manual creation of features, the next approach I want to discuss is a fully automated function for creating features. How often do you get sent plain sequences that have no features annotated, even though you know your favorite gene is on there, along with a few…
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101 things you (maybe) didn’t know about MacVector: #25 – Creating Features from Analysis Results
Read more: 101 things you (maybe) didn’t know about MacVector: #25 – Creating Features from Analysis ResultsIn my last post I described how you can quickly create and annotate features onto a DNA sequence, although the post was primarily aimed at users who are new to MacVector. In this post I’ll take a look at how you can quickly and easily annotate a DNA sequence with features based on the results…
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Season’s greetings from the MacVector team
Read more: Season’s greetings from the MacVector teamThere is no time more fitting to say “Thank You” and to wish you a Happy Holiday Season and a New Year full of health, happiness, and great results from your research. Make sure you put down that pipette and relax , and if you’ve not updated to MacVector 12.7, make sure you take time…
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101 things you (maybe) didn’t know about MacVector: #24 – Creating Features
Read more: 101 things you (maybe) didn’t know about MacVector: #24 – Creating FeaturesThis is a tip primarily aimed at new users of MacVector, but may be of interest to anyone who wants better understand the way MacVector handles features. MacVector can create wonderfully detailed graphical maps of a sequence, showing all the points of interest, restriction sites, open reading frames etc. However, each item to be displayed…
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101 things you (maybe) didn’t know about MacVector: #23 – Viewing Publications
Read more: 101 things you (maybe) didn’t know about MacVector: #23 – Viewing PublicationsIf you ever download sequences from Entrez, or open a GenBank formatted sequence file, you will often see PUBMED entries in the Annotations tab with blue colored numeric IDs; The blue color indicates that this is a link that you can use to retrieve an abstract of the publications. If you double-click on the PUBMED…
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Removing a restriction site by linearising and religating a plasmid
Read more: Removing a restriction site by linearising and religating a plasmidBeing able to remove a restriction site by digesting to linearise a plasmid, Klenow treating then religating the linear fragment is a very useful trick and an often requested feature. This is now possible with the new Cloning Clipboard in MacVector 12.7. select the enzyme recognition site you want to remove Click DIGEST to linearize…
