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101 things you (maybe) didn’t know about MacVector: #30 – Submitting Sequences To GenBank using Sequin
Read more: 101 things you (maybe) didn’t know about MacVector: #30 – Submitting Sequences To GenBank using SequinNote: while preparing this blog post we discovered a bug in MacVector 12.7.4 that prevents submission using the exact steps shown here. Be sure you are using MacVector 12.7.5 or later which has the bug fixed. If you are using an earlier version, send an e-mail to support@macvector.com and we’ll send you the details of…
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101 things you (maybe) didn’t know about MacVector: #29 – Option-Click to Close All Windows
Read more: 101 things you (maybe) didn’t know about MacVector: #29 – Option-Click to Close All WindowsIf you are like me, you often find yourself with many, many windows open while using MacVector. Sometimes you just want to get rid of everything and start all over again with a different project. You can always just quit MacVector and start again, but there is an easier way: Hold down the option key…
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101 things you (maybe) didn’t know about MacVector: #28 – Identifying Methylation Blocked Restriction Sites
Read more: 101 things you (maybe) didn’t know about MacVector: #28 – Identifying Methylation Blocked Restriction SitesA big thanks to Jeffrey Dvorin at Boston Children’s Hospital for this great suggestion. Most common laboratory strains of E. coli contain a number of methylase enzymes that modify DNA residues, preventing certain restriction enzymes from cutting DNA isolated from those strains. The two most relevant enzymes are the Dam methylase that methylates the A…
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101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson Assemblies
Read more: 101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson AssembliesWe’ve had a few MacVector users ask us recently if MacVector can be used to create constructs using the increasingly popular “Gibson Assembly” method. For those who are not familiar with this method, it was first described in 2009 by Daniel Gibson of the J. Craig Venter Institute. They showed that you can assemble multiple…
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MacVector 12.7 Training Workshop, Weatherall Institute for Molecular Medicine
Read more: MacVector 12.7 Training Workshop, Weatherall Institute for Molecular MedicineWhen: Tuesday, 23rd of April 2013, 14:00 until 16:00 Where: WIMM Seminar Room, WIMM Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last two years to MacVector.…
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MacVector 12.7 Training Workshop at Emory University
Read more: MacVector 12.7 Training Workshop at Emory UniversityWhen: Monday March 25, 1:00-3:00 pm Where: Calhoun Auditorium, Clinic B building “Tunnel Level” Dr. Kevin Kendall of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the last 2 years to…
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Using Blast and Entrez in MacVector with a proxy server
Read more: Using Blast and Entrez in MacVector with a proxy serverBlast and Entrez connect to the NCBI server using the normal “http” ports (exactly the same as if it was a web browser). If a web browser can access the NCBI’s server then MacVector should be able to. The address for the Blast server is: http://www.ncbi.nlm.nih.gov:80/blast/Blast.cgi However, if you get the following error message then…
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101 things you (maybe) didn’t know about MacVector: #26 – Creating Features Automatically
Read more: 101 things you (maybe) didn’t know about MacVector: #26 – Creating Features AutomaticallyFollowing on from my recent posts on manual and semi-manual creation of features, the next approach I want to discuss is a fully automated function for creating features. How often do you get sent plain sequences that have no features annotated, even though you know your favorite gene is on there, along with a few…
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101 things you (maybe) didn’t know about MacVector: #25 – Creating Features from Analysis Results
Read more: 101 things you (maybe) didn’t know about MacVector: #25 – Creating Features from Analysis ResultsIn my last post I described how you can quickly create and annotate features onto a DNA sequence, although the post was primarily aimed at users who are new to MacVector. In this post I’ll take a look at how you can quickly and easily annotate a DNA sequence with features based on the results…
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Season’s greetings from the MacVector team
Read more: Season’s greetings from the MacVector teamThere is no time more fitting to say “Thank You” and to wish you a Happy Holiday Season and a New Year full of health, happiness, and great results from your research. Make sure you put down that pipette and relax , and if you’ve not updated to MacVector 12.7, make sure you take time…
