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Tag: cloning

  • Simulated Agarose Gels

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    Chris
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    MacVector 14.5 has a Agarose Gel interface which allows you to view photo-realistic recreations of restriction digests of linear and circular DNA molecules. The gels look so realistic that users have had a hard time telling photos of their own digests from the simulation in MacVector. When you first use the new tool and compare…

  • Cloning Clipboard: A few tips on working with digested fragments

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    Chris
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    The Cloning Clipboard is an easy, and flexible, way to design and document your cloning strategies. Recently we discussed how every ligation in a cloning procedure is documented and stored in a sequence, so you always know how a construct was made. If you need to manipulate a fragment as a sequence, before ligating it,…

  • Cloning Clipboard: Documenting the history of a construct

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    Chris
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    Designing and documenting cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. Not only that but every step is documented and recorded in the resulting sequence, so you always know where each fragment came from. Ligating a single fragment into a vector is as…

  • MacVector Cloning Edition

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    Chris
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    Does your lab only need a simple DNA analysis application that will help you design primers and cloning experiments? Has your budget been cut to the bone? If the answer is a resounding “yes”, then we’ve released a new version of MacVector just for you. MacVector Cloning Edition provides all of the functionality you need…

  • Restriction enzyme sites and tooltips.

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    Chris
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    EDIT: April 12, 2019 From MacVector 16 and later the setting for the Automatic RE Analysis is in: PREFERENCES | SCAN DNA | RESTRICTION SITES Quickly viewing the recognition sequence and cut site of a restriction site is very easy in the Map tab. If you hover your mouse over a restriction site in the Map…

  • 101 things you (maybe) didn’t know about MacVector: #27 – Documenting Gibson Assemblies

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    Kevin
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    We’ve had a few MacVector users ask us recently if MacVector can be used to create constructs using the increasingly popular “Gibson Assembly” method. For those who are not familiar with this method, it was first described in 2009 by Daniel Gibson of the J. Craig Venter Institute. They showed that you can assemble multiple…

  • Removing a restriction site by linearising and religating a plasmid

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    Chris
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    Being able to remove a restriction site by digesting to linearise a plasmid, Klenow treating then religating the linear fragment is a very useful trick and an often requested feature. This is now possible with the new Cloning Clipboard in MacVector 12.7. A new circular sequence will be created from your digested fragment. The origin…

  • MacVector 12.7 is now out.

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    Chris
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    We’re pleased to announce that MacVector 12.7 has just been released. The most exciting new feature is the Cloning Clipboard. We’ve had a lot of user requests over the past few years for help with designing cloning operations and the user feedback we had during the beta testing of MacVector 12.7 makes us think we’ve…

  • MacVector 12.7 preview release

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    Chris
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    Our next release is MacVector 12.7 and it will be out very soon. However, if don’t want to wait then you can download a preview before the real release in November. MacVector 12.7 has a new Cloning Clipboard tool that dramatically simplifies the creation of new DNA constructs. The new functionality not only lets you…

  • Subcloning using the Cloning Clipboard

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    Chris
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    Cloning on MacVector has always been an easy procedure. Every digestion places a fragment on the cloning clipboard which then allows you to ligate that fragment into a vector. However, this limitation has now gone with the Cloning Clipboard. The Cloning Clipboard allows you to store a history containing fragments from previous digestions. Not only…