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Annotating primers to your sequence with MacVector
Read more: Annotating primers to your sequence with MacVectorDesigning a pair of primers to amplify a single feature is pretty quick with MacVector. Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer,…
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Use a right-click in the Contig Editor tab to see if your contig can be circularized
Read more: Use a right-click in the Contig Editor tab to see if your contig can be circularizedMacVector 16 incorporates no less than THREE different de novo assemblers, phrap, velvet and SPAdes. While all are great assemblers, with each having their own specific advantages, none of them will generate a circular sequence from input reads. However, MacVector 16 also includes a new feature to help you with this. If you are assembling…
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MacVector are at the ASCB – EMBO 2017 meeting in Philadelphia
Read more: MacVector are at the ASCB – EMBO 2017 meeting in PhiladelphiaThe MacVector team will be at the ASCB/EMBO 2017 meeting this coming Sunday. The conference starts on Sunday the 3rd of December and runs until Tuesday the 5th. It’s being held at the Pennsylvania Convention Center. Our booth is 1002. If you are in Philadelphia for the conference please pop along and say “hi!”. We…
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Downloading hits from the MacVector BLAST Map results tab
Read more: Downloading hits from the MacVector BLAST Map results tabMacVector’s BLAST Map results tab (added in MacVector 15.5) is a unique interface for examining the annotations around hits to a query sequence. Each pane in the display represents a High Scoring Segment Pair, as seen in the BLAST Aligned Sequence tab. At the lower left corner of each pane is a Download button –…
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Turning on/off the SCAN FOR missing Features and ORFs
Read more: Turning on/off the SCAN FOR missing Features and ORFsIf you’re running MacVector 15.5 or later then you will have noticed extra features annotated to your sequences. These are from the Scan for ORFs tool (added in MacVector 15.5) and the Scan For Missing Features (added in MacVector 16) tools that automatically scan every DNA sequence window for open reading frames, missing annotations and…
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Controlling The Automatic ORF Display in MacVector 15.5
Read more: Controlling The Automatic ORF Display in MacVector 15.5MacVector automatically scans every DNA sequence window for open reading frames and displays the results in the Map tab. The setting for this are controlled by the MacVector | Preferences -> DNA Map pane, along with the automatic Show restriction sites settings. The Minimum Number of Codons setting is fairly obvious. 5’ ends are starts…
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Use the BLAST Map to better identify blast hits
Read more: Use the BLAST Map to better identify blast hitsWith the advent of cheap Next Generation Sequencing technologies, there has been an explosion of whole genome sequences deposited in BLAST databases. One consequence of this is that, particularly for sequences of bacterial origin, most of the significant hits are to entire genomes. The classic BLAST results show the sequence alignments, but give no indication…
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MacVector’s Scan For Missing Features tool makes beautiful plasmid maps easy!
Read more: MacVector’s Scan For Missing Features tool makes beautiful plasmid maps easy!Scan for.. Missing Features: Sequences are automatically scanned and missing features displayed. A simple right-click converts them to a permanent feature. Even blank sequences will be displayed fully annotated with common features. You can even add your own proprietary features.
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101 things you (maybe) didn’t know about MacVector: #51 – Rapid assembly of genomes with Velvet and SPAdes
Read more: 101 things you (maybe) didn’t know about MacVector: #51 – Rapid assembly of genomes with Velvet and SPAdesNot so long ago, to assemble even a small genome with Next Generation Sequencing data required an array of clustered computers and a lot of patience. But improvements in algorithms and hardware mean that it is now realistic to assemble bacterial genomes, or even smaller eukaryotic genomes using MacVector on a modest laptop machine. MacVector…
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MacVector 16: Our latest release takes automatic sequence annotation to a whole new level
Read more: MacVector 16: Our latest release takes automatic sequence annotation to a whole new levelMacVector 16, our latest release, makes beautiful plasmid maps easier, and accurate de novo assembly achievable on your own desktop. Scan for.. Missing Features: Sequences are automatically scanned and missing features displayed. A simple right-click converts them to a permanent feature. Even blank sequences will be displayed fully annotated with common features. You can even…
