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MacVectorTip: How to Control the Length of Window Title

MacVectorTip: Assembling Nanopore or PacBio Long-Read Data with Flye

Handling Gaps in Multiple Sequence Alignment Consensus Calculations

Automating annotation of sequences via BLAST

MacVectorTip: Annotating and Comparing Genome Segments

MacVectorTip: Use Align to Folder to filter NGS data for specific genes

MacVectorTip: Restriction enzyme sites and tooltips

MacVectorTip: Advanced Align to Reference Editing

MacVectorTip: How to Toggle Between Single-Letter and Three-Letter Amino Acid Translation Code

MacVectorTip: Drag and drop in the Gibson Assembly window

MacVectorTip: organizing assemblies and sequencing datasets with the Assembly Project manager.

MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotype

MacVectorTip: Grayed out graphics indicate Missing Features

MacVectorTip: Selecting the sequence from a single restriction enzyme site to the end of a linear sequence

MacVectorTip: How to Customize the Toolbars of MacVector windows

MacVectorTip: macOS Dark Mode and forcing MacVector to open in Light Mode

MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translation

macOS’s tabbed windows and MacVector

MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotype

MacVectorTip: correctly flagging PacBio and Oxford Nanopore datasets for assembly by Flye

MacVectorTip: Assembling Fungal Genomes using SPAdes

MacVectorTip: Viewing external database entries for features in a sequence.

MacVectorTip: How to Customize Window Button Toolbars

MacVectorTip: Understanding Color Groups

MacVectorTip: Scan For… Missing Primers: Automatically display Primer Binding Site on your sequences

MacVectorTip: Viewing genotype changes in Align to Reference assemblies

Calculating the optimal PCR annealing temperature

How to copy a specific short amino acid translation of a sequence

Optimizing Reverse Translations

Use Database | Auto-Annotate Sequence to annotate prokaryotic genomes

Which DNA Matrix to use in Align To Folder?

Gap closing and genome finishing tools in Align to Reference and Assembler.

Use a right-click in the Editor tab to see if your contig can be circularized

Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features

Opening Genbank or FASTA files with multiple sequences as individual sequences

Optimizing Align To Folder Parameters for use with NGS Data

HOW DO I video guides to common molecular biology workflows

Show or hide the RE Picker and the Graphics Palette from the toolbar

Scan For… Missing Primers: Automatic Primer Binding Site Display

Restoring file associations when MacVector no longer opens your sequence documents.

How to find one-out Restriction Sites that will change a protein coding sequence

Seasons Greetings from the MacVector Team

Use Database | Auto-Annotate Sequence to annotate prokaryotic genomes

Automatically displaying open reading frames with MacVector’s Scan For Open Reading Frames tool

Pasting tabular data from MacVector into Microsoft Excel

Using MacVector’s Agarose Gel tool to design a digest to screen minipreps after a ligation.

RNASeq Expression Analysis with MacVector and Assembler

How to split large fastq files for more manageable assemblies

Balancing Velvet KMER and coverage

Use a right-click in the Editor tab to see if your contig can be circularized

Assemble bacterial genomes in minutes on your Mac laptop

Simple Assembly of Sanger Sequencing Files with MacVector Assembler

Use the Replica Button For Synchronized Views

How to Identify Bacterial Promoters Using MacVector

Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features

Opening multiple sequences as alignments or individual sequences

RNASeq Expression Analysis with NGS data

Use a right-click in the Contig Editor tab to see if your contig can be circularized

Downloading hits from the MacVector BLAST Map results tab

Controlling The Automatic ORF Display in MacVector 15.5

Use the BLAST Map to better identify blast hits

Downloading hits from the MacVector 15.5 BLAST Map results tab

Controlling Automatic ORF Display

Use the BLAST Map to better identify blast hits

Generating a primer report to send to your Oligo Synthesis service

How to toggle between 1 and 3 letter amino acid codes

Clone construction using Digest/Ligate rather than Copy/Paste

Viewing external database entries for features in a sequence.

Tear-off Result Window Tabs: make viewing results easier.

Use the Edit | Transformations menu to change the case of sequence residues

Using BLAST to automatically annotate a sequence

Use the BLAST Map to better identify blast hits

Controlling The Automatic ORF Display in MacVector 15.5

Clone construction using Digest/Ligate rather than Copy/Paste

Simple but accurate restriction enzyme based clone construction using Copy and Paste

How to change the default appearance of features

Use File->Export in the Contig Editor to save selected reads

Use File->Export to save sequences in a different file format

How to reset the graphics palette if it disappears

Tips for finding ORFs in your sequence

Using QuickTest Primer to check for hairpins in sequences, and not just primers.

Download the latest published version of your favorite sequence with its accession number

Customizing BLAST alignment results to make mismatches more noticeable

How to save contig consensus sequences from assembly projects

Search fastq files and retrieve matching reads into paired fastq files

How to retrieve BLAST hits from the Aligned Sequences result tab

Displaying CDS features as translations in the Map tab.

Screening for CRISPR Indels using Align To Reference

How to align DNA sequences based on their amino acid translations

Functional domain analysis of protein sequences using InterProScan

Create your own Primer Database.nsub from an Excel spreadsheet

Use Analyze->Primer Database Search to scan sequences for primer binding sites

Using the Primer Database to store your lab’s collection of primers

Viewing external database entries for features in a sequence.

Optimizing the Reverse Translation function

Tear-off Result Window Tabs: make viewing results easier.

Highlighting sequence using color and lower case in the Editor tab

Restriction enzyme analysis in MacVector and REBASE

Drag and drop to quickly annotate ORFs

Download the latest published version of your favorite sequence with its accession number

Workflows

  • Primer Design
  • Cloning
  • Sequence Assembly
  • CRISPR
  • Gibson Assembly

Assembly

  • Sequence Assembly
  • Genome finishing
  • Comparison with Sequencher

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