The Automatic Restriction Enzyme Analysis tool that is displayed on the sequence in the Map tab is very powerful. It automatically displays a custom set of restriction enzyme recognition sites on every sequence that you open. Unique recognition sites are displayed in red and sites with two or more cut locations are displayed in blue. […]
If you are looking for compatible cloning sites for a fragment, MacVector has a simple color-coded function to identify potential target sites in a vector. First select the fragment you wish to clone by clicking on two enzymes in the Map view of a single sequence document or from a restriction enzyme search result. Then […]
Quickly viewing the recognition sequence and cut site of a restriction site is very easy in the Map tab. If you hover your mouse over a restriction site in the Map tab, a tooltip will show you the restriction enzyme recognition site, the location of the cut site, and number of times that enzyme cuts […]
It’s quite a few months since the last update on the development of MacVector for Windows. Work is progressing very well and a lot of the underlying functionality is now finished. The Cloning Clipboard, Restriction Enzyme analysis and QuickTest Primer tools are all done. The release is still some time away, but if you are […]
The most simple way of generating new constructs in MacVector using restriction enzyme sites is to: [shift]-select two sites in the Map tab of a source molecule. Switch to the Map tab of a target vector. Select the corresponding site(s) there. Simply choose Edit | Paste to insert the copied fragment into the target molecule. […]
We’ve previously discussed how every ligation is documented. You get a “Frag” annotation that contains the date, source sequence, enzymes used and any end modification that was done to that fragment during the ligation. However, we had regular requests to make it easier for user to document their constructs in other ways. For example being […]
MacVector 14.5 has a Agarose Gel interface which allows you to view photo-realistic recreations of restriction digests of linear and circular DNA molecules. The gels look so realistic that users have had a hard time telling photos of their own digests from the simulation in MacVector. When you first use the new tool and compare […]
The Cloning Clipboard is an easy, and flexible, way to design and document your cloning strategies. Recently we discussed how every ligation in a cloning procedure is documented and stored in a sequence, so you always know how a construct was made. If you need to manipulate a fragment as a sequence, before ligating it, […]
Designing and documenting cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. Not only that but every step is documented and recorded in the resulting sequence, so you always know where each fragment came from. Ligating a single fragment into a vector is as […]
Does your lab only need a simple DNA analysis application that will help you design primers and cloning experiments? Has your budget been cut to the bone? If the answer is a resounding “yes”, then we’ve released a new version of MacVector just for you. MacVector Cloning Edition provides all of the functionality you need […]