General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

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A few tips on working with digested fragments in the Cloning Clipboard

Annotating the sequence in the Editor tab

Assemble bacterial genomes in minutes on your Mac laptop

Automatically displaying open reading frames with MacVector’s Scan For Open Reading Frames tool

Balancing Velvet KMER and coverage

Calculating the melting temperature of PCR primers

Calculating the optimal PCR annealing temperature

Calculating the PCR annealing temperature for a pair of primers

Changing the sequence alignment match and mismatch characters

Clearing the history in the Primer and Find dialogs

Clone construction using Digest/Ligate rather than Copy/Paste

Clone construction using Digest/Ligate rather than Copy/Paste

Controlling Automatic ORF Display

Controlling The Automatic ORF Display in MacVector 15.5

Controlling The Automatic ORF Display in MacVector 15.5

Controlling the realism of Agarose Gels

Create constructs using the Cloning Clipboard

Create your own Primer Database.nsub from an Excel spreadsheet

Creating a custom set of restriction enzymes containing just the enzymes in your freezer drawer.

Creating cloning construction flowcharts in third party applications

Customizing BLAST alignment results to make mismatches more noticeable

Designing and documenting cloning strategies

Displaying CDS features as translations in the Map tab.

Displaying CDS features as translations in the Map tab.

Displaying features in the Editor tab

Download the latest published version of your favorite sequence with its accession number

Download the latest published version of your favorite sequence with its accession number

Downloading BLAST Hits from the NCBI

Downloading BLAST Hits from the NCBI direct to your desktop

Downloading hits from the MacVector 15.5 BLAST Map results tab

Downloading hits from the MacVector BLAST Map results tab

Drag and drop to quickly annotate ORFs

Editing the appearance of your sequence maps

Functional domain analysis of protein sequences using InterProScan

Gap closing and genome finishing tools in Align to Reference and Assembler.

Generating a primer report to send to your Oligo Synthesis service

Generating a primer report to send to your Oligo Synthesis service

Highlighting sequence using color and lower case in the Editor tab

HOW DO I video guides to common molecular biology workflows

How to align DNA sequences based on their amino acid translations

How to automatically annotate bare sequences

How to change the circular origin of a sequence

How to change the default appearance of features

How to change the default appearance of RE sites

How to copy a specific short amino acid translation of a sequence

How to display and manipulate segmented Features

How to display translations in the sequence editor

How to find and open all the sequences on your computer containing a specific gene.

How to find one-out Restriction Sites that will change a protein coding sequence

How to Identify Bacterial Promoters Using MacVector

How to identify methylation blocked Restriction Sites

How to import annotated GenBank sequences via copy and paste

How to increase the number of graphics levels to stop features overlapping.

How to increase the number of graphics levels to stop features overlapping.

How to make feature arrows point in the other direction

How to remove gaps from a sequence

How to reset the graphics palette if it disappears

How to reset the numbering after pasting a DNA fragment

How to reset the sequence numbering when working with a subsection from a larger sequence

How to reset the zoom level when you Drag Zoom into your sequence.

How to retrieve BLAST hits from the Aligned Sequences result tab

How to save contig consensus sequences from assembly projects

How to select all identical restriction enzyme sites

How to split large fastq files for more manageable assemblies

How to toggle between 1 and 3 letter amino acid codes

How to toggle between 1 and 3 letter amino acid codes

How to use Codon Preference plots

Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features

Import Multi-Sequence Genbank Files into an Assembly Project for easy access to Features

Importing sequences/features from websites such as ENSEMBL or UCSC’s Genome Browser

Keep up to date with MacVector auxiliary files

Know Your Alignments

macOS’s tabbed windows and MacVector

MacVector’s compatibility with different versions of macOS/OS X

MacVectorTip: Assembling Fungal Genomes using SPAdes

MacVectorTip: correctly flagging PacBio and Oxford Nanopore datasets for assembly by Flye

MacVectorTip: Create custom Codon Usage Tables for ORF analysis and reverse translation

MacVectorTip: How to Customize Window Button Toolbars

MacVectorTip: Identifying, Selecting and Assembling NGS reads with a variant genotype

MacVectorTip: macOS Dark Mode and forcing MacVector to open in Light Mode

MacVectorTip: Scan For… Missing Primers: Automatically display Primer Binding Site on your sequences

MacVectorTip: Understanding Color Groups

MacVectorTip: Viewing external database entries for features in a sequence.

MacVectorTip: Viewing genotype changes in Align to Reference assemblies

Opening Genbank or FASTA files with multiple sequences as individual sequences

Opening matching sequences from an Align To Folder search

Opening multiple sequences as alignments or individual sequences

Optimizing Align To Folder Parameters for use with NGS Data

Optimizing Reverse Translations

Optimizing the Reverse Translation function

Organising your sequences using Smart Folders

Pasting tabular data from MacVector into Microsoft Excel

Print Agarose Gel images with black bands on a white background

Quick shortcuts to zoom in and out in the Single Sequence Map tab

Quickly checking a small sequencing project

Quickly design a pair of primers to amplify a feature

Removing a restriction site from a plasmid with the Cloning Clipboard

Restoring file associations when MacVector no longer opens your sequence documents.

Restriction enzyme analysis in MacVector and REBASE

Restriction enzyme sites and tooltips