Author Archives: Chris

CRISPR tools in MacVector 18

If you are using CRISPR editing techniques then there are two useful functions in MacVector for you: MacVector will scan Nucleic Acid sequences for Protospacer Adjacent Motifs (PAM) associated with the CRISPR Cas9 and related enzymes cleavage and modification functions. MacVector’s Analyze | Align To Reference… tool is ideal for screening reads for the short […]

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Using the RE Picker to display restriction enzyme sites in your insert that do not cut your vector.

It’s generally useful with restriction enzyme based cloning to know what restriction enzymes will cut your vector and not cut your insert, or vice versa. There are a few ways to accomplish this with MacVector, but a useful way is to prepare a file of enzymes that do not cut your vector. This is especially […]

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MacVector, Apple Silicon and macOS Big Sur compatibility

Over twenty years ago Apple called an end to the Classic Mac with the release of OS X, followed five years later by the transition from PowerPC to Intel. A major step forward in the Macintosh world. Earlier this year Apple announced the next stage in the Apple world, Apple Silicon Macs and macOS Big […]

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Virtual Gene Cloning from NGS RNA-Seq Data

The NCBI Sequence Read Archive (SRA) database is a huge resource of Next Generation Sequencing experimental data. Many groups and laboratories deposit data here that they have generated for their own specific projects that can be datamined for other unrelated projects with a minimum of effort. MacVector contains a number of powerful tools that can […]

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Working From Home: An overview of primer design workflows in MacVector.

Working from home?. We want to help familiarize you with the wide range of functionality in MacVector that you may never have used before. Here’s an overview of workflows for designing, testing, documenting and storing primers. You may not have a PCR machine on the kitchen table, but why not take the time to store […]

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Working from home: An overview of assembling sequence data with MacVector and Assembler

Working from home Here’s a series of blog posts on the wide range of functionality in MacVector that you may never have used before. This is an overview of the many different sequence assembly tools within MacVector. The MacVector team used these tools to mine existing sequencing archives to assemble a new Pangolin SARS-CoV–2 genome. To […]

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Working From Home: accessing video tutorials of common workflows inside MacVector

During the Covid-19 pandemic we want to ensure that you have access to the MacVector license that you would use in the lab, if you are working from home. If you use MacVector, even an older version, and are having trouble activating it (or installing it) at home email MacVector Support and we will help. If you […]

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Working from home – Getting your sequence into MacVector

During the Covid-19 pandemic we want to ensure that you have access to the MacVector license that you would use in the lab, if you are working from home. If you use MacVector, even an older version, and are having trouble activating it (or installing it) at home email MacVector Support and we will help. If you […]

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Working From Home – common workflows

During the Covid-19 pandemic we want to ensure that you have access to the MacVector license that you would use in the lab, especially if you are working from home. If you use MacVector, even an older version, and are having trouble activating it (or installing it) at home email MacVector Support and we will help. If […]

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Working From Home – Comparing sequences

During the Covid-19 pandemic we want to ensure that you have access to the MacVector license that you would use in the lab, especially if you are working from home. If you use MacVector, even an older version, and are having trouble activating it (or installing it) at home email MacVector Support and we will help. If […]

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