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Cloning Clipboard: A few tips on working with digested fragments
The Cloning Clipboard is an easy, and flexible, way to design and document your cloning strategies. Recently we discussed how every ligation in a cloning procedure is documented and stored in a sequence, so you always know how a construct was made. If you need to manipulate a fragment as a sequence, before ligating it, […]
101 things you (maybe) didn’t know about MacVector: #48 – Setting the Alignment match/mismatch characters
There are many output windows throughout MacVector that display aligned sequences. If you run an Align To Folder, Create Dotplot or Internet Blast Search, one or more of the output windows will show alignments in a plain text format with some sort of character indicating matching residues. The default is to use the vertical | […]
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How Assembler uses quality scores to create assemblies
A common problem with all types of sequence assembly is distinguishing between sequencing errors and true genomic variations. Quality scores are one way to help the algorithm identify if a variation is of high quality and therefore likely to be a SNP or a sequencing error. For Assembler trace files can be basecalled with Phred, […]