Author Archives: Chris

Cloning Clipboard: A few tips on working with digested fragments

The Cloning Clipboard is an easy, and flexible, way to design and document your cloning strategies. Recently we discussed how every ligation in a cloning procedure is documented and stored in a sequence, so you always know how a construct was made. If you need to manipulate a fragment as a sequence, before ligating it, […]

Posted in Tips | Tagged | Comments closed

How Assembler uses quality scores to create assemblies

A common problem with all types of sequence assembly is distinguishing between sequencing errors and true genomic variations. Quality scores are one way to help the algorithm identify if a variation is of high quality and therefore likely to be a SNP or a sequencing error. For Assembler trace files can be basecalled with Phred, […]

Posted in Algorithms, Tips | Tagged , , , | Comments closed

Visualising ORF analysis results in the MAP tab.

The Map tab of MacVector is a powerful way to visualise and interact with your sequences. All analysis tools will work directly in the Map tab. You can design primers, ligate and digest fragments from the Cloning Clipboard, visualise translated CDS regions and much more. In fact one of the only tasks, you will need […]

Posted in Tips, Uncategorized | Tagged , | Comments closed

Cloning Clipboard: Documenting the history of a construct

Designing and documenting cloning strategies is easy with the Cloning Clipboard. You can perform quite complex ligations by simply dragging compatible ends together. Not only that but every step is documented and recorded in the resulting sequence, so you always know where each fragment came from. Ligating a single fragment into a vector is as […]

Posted in Techniques, Tips | Tagged , | Comments closed

MacVector 14.0.4 is OS X 10.11 El Capitan compatible.

OS X 10.11 El Capitan was released last week. We’ve been testing MacVector on the developer releases of OS X EL Capitan in the run up to the official release. However, as usual we prefer to fully test on an official release of OS X before saying that MacVector is supported. We’ve now done so […]

Posted in Releases | Tagged , | Comments closed

Buy two copies of MacVector 14 for the price of one.

For the month of October if you buy a personal or standard license of MacVector Pro then you’ll get one free! We never stop working to keep MacVector your best solution for designing primers, subcloning into vectors, aligning your sequences and checking small sequencing projects on the Mac. So don’t miss this chance to start […]

Posted in Releases | Tagged , | Comments closed

Using the Primer Database to store your lab’s collection of primers

MacVector now directly supports storing primers within a primer database. The Primer Database tool allows you to save and retrieve primers from subsequence files within Primer3 and Quicktest Primer. You can easily create your own primer database, use existing files or import primers from Excel. Many users use subsequence files to store primers, and in […]

Posted in Releases, Tips | Tagged , , | Comments closed

Editing the appearance of individual sequences maps

Although we think that the default appearance of sequence maps in MacVector is very pretty, sometimes the defaults are not to everybody’s taste! If you think this way, then changing how maps look is very easy. Rather than edit the appearance of all of your sequences it is far better to modify the default symbol […]

Posted in Tips | Tagged | Comments closed

MacVector Cloning Edition

Does your lab only need a simple DNA analysis application that will help you design primers and cloning experiments? Has your budget been cut to the bone? If the answer is a resounding “yes”, then we’ve released a new version of MacVector just for you. MacVector Cloning Edition provides all of the functionality you need […]

Posted in Releases | Tagged , , , | Comments closed

MacVector for Windows update

The development for MacVector for Windows progresses. It’s not ready for release yet but it’s definitely the last stage before testing. MacVector for Windows is not a Java version of MacVector. MacVector, the original, is designed for, and fully integrates with, the OS X environment. We know users really appreciate that. If you can use […]

Posted in Releases | Tagged , | Comments closed