Category Archives: Techniques

Howtos, tips and descriptions of various techniques for using MacVector.

Quickly checking a small sequencing project

For analysing large sequencing datasets, whether de novo or mapping reads against a reference you need Assembler. However, many times you do not need a powerful tool but just a quick way to check some sequencing data. For example for checking small sequencing projects, such as a site directed mutagenesis, looking for SNPs in a […]

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MacVector 12.7 Training Workshop, LMB, Cambridge

When: Thursday 19th September, 2013, 2:00 – 4:00, Where: Max Perutz Lecture Theatre, LMB Chris Lindley of MacVector, Inc. will be giving a workshop for both novice and advanced users of MacVector, reviewing both basic and advanced functions in MacVector. In particular, he will highlight the new functionality introduced over the past two years to MacVector. […]

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Subcloning using the Cloning Clipboard

Cloning on MacVector has always been an easy procedure. Every digestion places a fragment on the cloning clipboard which then allows you to ligate that fragment into a vector. However, this limitation has now gone with the Cloning Clipboard. The Cloning Clipboard allows you to store a history containing fragments from previous digestions. Not only […]

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QuickTest Primer: Designing Primers in MacVector

MacVector has a brand new tool for designing primers. QuickTest Primer completely changes the way primers can be designed on a computer. It simplifies primer design by showing your primer and its statistics in realtime. Does your primer have a hairpin? Nudge it along your template until the hairpin goes? Want to add a restriction […]

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Importing features from a Genome Browser

(updated March 21, 2018) MacVector’s Import Features tool allows you to import annotation from many Genome Browsers (e.g. Ensemble, UCSC, etc). MacVector can annotate an empty or annotated sequence. BED, GFF, GTF, and GFF3 formats GFF, GTF, GFF3 & BED files are all file formats that are used to store annotation (features) generally without containing any […]

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Showing features as bases or a translation in a plasmid map

Everybody has different tastes and giving everybody identical plasmid maps is unfair! So MacVector is designed to be as flexible as possible to allow you to make your maps look like YOU want then to look. In this theme was a recent change where appropriate features can be shown as residues when there is sufficient […]

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MacVector 12.5: Creating alignments with T-Coffee and Muscle

With MacVector 12.5 we’ve added additional multiple sequence alignment (MSA) algorithms. Muscle and T-coffee have been added to the Multiple Sequence Alignment editor complementing the existing ClustalW algorithm. We’ve wanted both of these for a while now and judging from the results of last year’s survey so have many users. Both T-Coffee and Muscle are […]

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MacVector 12.5: Sequence Assembly made easy.

This is part of a series of posts about and leading up to the release of MacVector 12.5. Assembler has always made it easy to assemble your sequencing projects. It hides the complicated algorithms and provides a point and click interface to show you the results. With the release of MacVector 12.5 the range of […]

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Zooming to the sequence level in the Map view

When zoomed to the residue level it’s easy to check restriction enzyme ends.   Technorati Tags: MacVector  

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Label customisation using metatags in the Map View

MacVector has always given the user great control in being able to customise the map’s appearance. By default the label will show the full text description of a feature and all its qualifiers (see the first metatag in the list below). However, the label can contain whatever text you want it to display. Obviously you […]

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