MacVectorTip: How to Toggle Between Single-Letter and Three-Letter Amino Acid Translation Code

Many views in MacVector display amino acid translations above or below DNA sequences. Typically, these are from CDS features, but can also be the 3/6 frame translation of a sequence. You can display the amino acids as either the single-letter code, or as the three-letter code. You can toggle the setting using the MacVector | Preferences -> […]

Posted in Tips, Uncategorized | Also tagged , | Leave a comment

MacVectorTip: displaying CRISPR PAM Sites on a sequence

CRISPR-Cas9 genetic editing mechanisms require a short (typically 20nt) RNA sequence complementary to a target site next to a Protospacer Adjacent Motif (PAM) sequence. The most commonly used Cas9 nuclease (SpCas9) from Streptococcus pyogenes recognizes the PAM sequence NGG. One new function introduced a few years ago will search for PAM sequences in your sequence. […]

Posted in Tips | Also tagged | Comments closed

MacVectorTip: visualizing shared domains in a protein alignment

MacVector has a domain-outlining facility for multiple sequence alignments, letting you easily visualize the relationships between features in aligned protein sequences. MacVector’s new multiple alignment file format retains the features/annotations from the sequences that are used to create the alignment. The colors of features from the individual sequence documents are used to outline the domains […]

Posted in Tips | Also tagged , | Comments closed

Tear-off Result Window Tabs: make viewing results easier.

All analysis results for an individual sequence are collected into a single tabbed result window to reduce window clutter. However, there are times when it is very convenient to have results displayed in side-by-side windows. For example, if you run a dot plot you can zoom in to view sections of the comparison by drag-selecting […]

Posted in Tips | Also tagged , | Comments closed