MacVectorTip: Designing Primers for Gibson Assembly

You can use MacVector to design primers for multi-fragment Gibson Assembly, and also generate the predicted recombinant DNA molecule resulting from the assembly. All you have to do to get started is choose the File->New->Gibson/Ligase-independent Assembly… menu item. From there, you can choose the type of assembly (it doesn’t have to be the usual 5’ […]

Posted in Tips | Also tagged | Leave a comment

What can MacVector do for my lab?

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Compare Genomes: Compares two related annotated genomes to identify identical, similar and weakly similar features. Sequence assembly of NGS data […]

Posted in Releases | Also tagged , , | Comments closed

Designing primers for Gibson Assembly with MacVector 17

MacVector 17 has a completely new tool for automated design of ligase-independent cloning strategies. The tool supports 5’ exonuclease driven Gibson assembly as well as the T4 DNA Polymerase 3’ exonuclease “Ligase Independent Cloning” approach. MacVector can automatically design primers when you specify fragments and vectors to use. You can provide custom primers (manually or […]

Posted in Releases, Techniques | Also tagged , , | Comments closed