General musings from the MacVector team about sequence analysis, molecular biology, the Mac in general and of course your favorite sequence analysis app for the Mac!

Tag Archives: pcr

Aligning primers against a template sequence

A common request, especially in our recent survey, is to align existing primer sequences against a template sequence. There are many ways to do this in MacVector, depending on what your requirements are Using the Find dialog For quickly finding a single primer in a sequence the Find dialog is the first point of call. [...]

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Creating your own subsequence library of primers

As mentioned in a recent post MacVector has a powerful feature called Subsequence searches. This function allows you to keep a library of sequence pattern matches, using a powerful nomenclature with up to three parts, and quickly scan protein and nucleic acid sequences with this library. .Although MacVector ships with a number of collections of [...]

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Designing and testing primers in MacVector – part 1

MacVector has many different primer tools for different purposes. In this, the first of a series of articles I want to present an overview of primer design in MacVector, and when it’s best to use which tool. I’ll also talk about what’s coming next in future releases. Design Primers (Primer3) The most flexible tool was [...]

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