What can MacVector do for my lab?

By Chris | Published: April 25, 2019

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Compare Genomes: Compares two related annotated genomes to identify identical, similar and weakly similar features. Sequence assembly of NGS data […]

Posted in Releases | Also tagged , , | Leave a comment

Workflows on designing, testing and storing primers in MacVector

By Chris | Published: June 4, 2018

MacVector has many primer tools to make designing, analyzing and cataloging your primers easy. Here are a few typical workflows. Designing primers Amplifying a gene You can design a set of primers to amplify a gene in as little as three mouse clicks. Open your sequence. Open the MAP view, and click on a feature. […]

Posted in Techniques, Tips | Also tagged | Comments closed

What can MacVector do for me?

By Chris | Published: April 5, 2018

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Sequence assembly of NGS data against a reference genome or compare your sequencing against your new construct. Translated Multiple Sequence […]

Posted in General | Also tagged , , , | Comments closed

Adding a primer to MacVector’s Primer Database.

By Chris | Published: January 10, 2018

  MacVector’s Primer Database tool makes it easy to store your lab’s regular primers. It allows you to save and retrieve primers from the Primer Database from the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites. The Primer Database will also store tails and allow you to specify […]

Posted in Tutorials | Also tagged , | Comments closed

Annotating primers to your sequence with MacVector

By Chris | Published: December 8, 2017

Designing a pair of primers to amplify a single feature is pretty quick with MacVector. Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer, […]

Posted in Uncategorized | Also tagged , , | Comments closed

Testing pairs of primers with MacVector

By admin | Published: November 3, 2017

MacVector’s Primer Design tools makes it easy to test your primers. You can insert them directly from MacVector’s Primer Database or copy and paste them. Tweet

Posted in Tips | Also tagged , | Comments closed

A whole new way to design primers with MacVector’s Quicktest Primer

By admin | Published: October 25, 2017

QuickTest Primer completely changes the way primers can be designed on a computer. It simplifies primer design by showing your primer and its statistics in realtime. Does your primer have a hairpin? Nudge it along your template until the hairpin goes? Want to add a restriction site? Then add one and again nudge your primer […]

Posted in Tutorials | Also tagged , | Comments closed

What can MacVector do for your lab?

By Chris | Published: September 12, 2017

Here’s an overview of what MacVector can do for you! Comparing sequences Whatever alignment your sequence needs, MacVector has the right tool. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Multiple sequence alignment of protein or DNA sequences using CLustalW/Muscle or T-Coffee and produce publication quality alignments. Sequence assembly of […]

Posted in Tips | Also tagged , | Comments closed

Generating a primer report to send to your Oligo Synthesis service

By Chris | Published: July 31, 2017

QuickTest Primer is a great tool for primer design. Paired with Primer Design/Test (Pairs) it gives you great control and flexibility for designing primers with tails, mismatches, silent mutations, one out sites and more. Once you’ve designed your primer the next step is to get it synthesized. QuickTest Primer will produce a PDF report of […]

Posted in Tips | Also tagged , | Comments closed

Using QuickTest Primer to check for hairpins in sequences, and not just primers.

By Chris | Published: March 13, 2017

Although QuickTest Primer is intended for designing primers, the interface is very flexible. If your sequence is not too long, you can use the Quickest Primer interface to scroll through a sequence and visually look for hairpins appearing in the hairpin pane. The easiest way to do this is to select the first ~100 nt […]

Posted in Tips | Also tagged , | Comments closed