Primer validation with MacVector: Primer3, Covid19 and primer design

By Chris | Published: March 16, 2020

The CDC recently published diagnostic real-time primers for identification of SARS-CoV–2 in any person suspected of having COVID–19. Unfortunately as pointed out on the Biome Informatics blog these primers have issues that should have easily been detected had the primers been tested using a good quality primer testing tool (the linked blog post uses Primer3). […]

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Calculating the optimal PCR annealing temperature

By Chris | Published: January 10, 2020

MacVector has several tools to help with primer design and testing. The Analyze | Primer Design/Test (Pairs) function uses the popular Primer3 algorithm to find suitable pairs of primers to amplify specified segments of DNA. You can also enter pairs of pre-designed primers and test their suitability for use in PCR. In both cases, the […]

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What can MacVector do for my lab?

By Chris | Published: April 25, 2019

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Compare Genomes: Compares two related annotated genomes to identify identical, similar and weakly similar features. Sequence assembly of NGS data […]

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Workflows on designing, testing and storing primers in MacVector

By Chris | Published: June 4, 2018

MacVector is the best PCR Primer design application on macOS. It has many innovative primer tools to make designing, analyzing and cataloging your primers or oligos easy. Here are a few typical workflows. Designing primers Amplifying a gene You can design a set of primers to amplify a gene in as little as three mouse […]

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What can MacVector do for me?

By Chris | Published: April 5, 2018

Here’s what MacVector can do for your lab. Comparing sequences Whatever type of alignment your sequence needs, there’s a tool in MacVector. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Sequence assembly of NGS data against a reference genome or compare your sequencing against your new construct. Translated Multiple Sequence […]

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Adding a primer to MacVector’s Primer Database.

By Chris | Published: January 10, 2018

  MacVector’s Primer Database tool makes it easy to store your lab’s regular primers. It allows you to save and retrieve primers from the Primer Database from the Primer3 and Quicktest Primer tools. You can also scan sequences for potential primer binding sites. The Primer Database will also store tails and allow you to specify […]

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Annotating primers to your sequence with MacVector

By Chris | Published: December 8, 2017

Designing a pair of primers to amplify a single feature is pretty quick with MacVector. Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer, […]

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Testing pairs of primers with MacVector

By admin | Published: November 3, 2017

MacVector’s Primer Design tools makes it easy to test your primers. You can insert them directly from MacVector’s Primer Database or copy and paste them. Tweet

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A whole new way to design primers with MacVector’s Quicktest Primer

By admin | Published: October 25, 2017

QuickTest Primer completely changes the way primers can be designed on a computer. It simplifies primer design by showing your primer and its statistics in realtime. Does your primer have a hairpin? Nudge it along your template until the hairpin goes? Want to add a restriction site? Then add one and again nudge your primer […]

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What can MacVector do for your lab?

By Chris | Published: September 12, 2017

Here’s an overview of what MacVector can do for you! Comparing sequences Whatever alignment your sequence needs, MacVector has the right tool. CRISPR Indel Analysis: Identify insertions and deletions following CRISPR editing of a target. Multiple sequence alignment of protein or DNA sequences using CLustalW/Muscle or T-Coffee and produce publication quality alignments. Sequence assembly of […]

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